Universal Human IgG/IgM Lateral Flow Serology Kit (Gold)
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Antibody tests are a method of choice to determine if a person has been exposed to a pathogen or not. They are also incredibly valuable in the detection of autoantibodies that can be found in human autoimmune disorders. In this test, a biotinylated antigen (User supplied) is mixed with a biotinylated rabbit IgG (bind to goat anti rabbit control line) and sample (human sera or plasma) is simply mixed into with the specially designed assay running buffer in a well of the supplied 96-well plate, mixed and is then added to the sample port of the cassette. Generally, the reaction is complete in 10-15 minutes. It is very important to note that the relative stoichiometry between the biotinylated antigen, biotinylated rabbit IgG added, and the streptavidin gold is critical for assay optimization. The appropriate concentration of biotinylated antigen to use with strips is dependent upon the purity and sequence and a standard curve can be used to determine the relative ratio (generally between 1ng-100ng per test). A positive control line (biotin-rabbit IgG) antibody will bind to the goat anti rabbit (GAR) line on the test to ensure the assay is running appropriately.
Attogene’s Human IgG/IgM universal lateral flow assay kit is a ready-to-use, universal test strip, which is based on the lateral flow technology that uses gold particles containing streptavidin to conveniently capture biotinylated antigens. The device is designed to easily develop qualitative or quantitative rapid test systems for detection of anti-human IgG and IgM antibody that react to the any antigen that can be biotinylated (i.e. viral antigen, autoimmune antigen) and is easily customizable providing every laboratory with the possibility to perform assay feasibility.
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IVD5412-F-96 MagPure Viral DNA/RNA Precast Kit
Product Info
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Product Info
Introduction
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main Functions
IVD5412 precast kit for kingfisher Flex
Applications
RT-PCR，PCR，NGS
Products
Viral DNA / RNA, body cell DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technolog
Process method
Manual or automatic
Sample type
Sample amount
200μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
Cat.No
Reagent
IVD5412-F-96
PK/Carrier RNA
1x 24 mg/Bottle
Protease Dissolve Buffer Blue
1.8 ml/Bottle
Tip
1
Sample Plate (DW Plate)
500µl Buffer MLB/Well
1
Wash 1 Plate (DW Plate)
500µl Buffer MW1/Well
1
Wash 2 Plate (DW Plate)
500µl Buffer CW/Well
1
Beads Plate (DW Plate)
500µl CW & 20µl MPN/Well
1
Elute Plate (KF Plate)
90µl NFW/Well
1
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Document
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
PACE Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE compatible genotyping assays are comprised of two competitive allele specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer. PACE Genotyping Master Mix is supplied at 2x concentration and with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.
Genotyping assay designs are available from 3CR Bioscience through our free PACE assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
Document
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
