Universal Human IgG/IgM Lateral Flow Serology Kit (Gold)
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Antibody tests are a method of choice to determine if a person has been exposed to a pathogen or not. They are also incredibly valuable in the detection of autoantibodies that can be found in human autoimmune disorders. In this test, a biotinylated antigen (User supplied) is mixed with a biotinylated rabbit IgG (bind to goat anti rabbit control line) and sample (human sera or plasma) is simply mixed into with the specially designed assay running buffer in a well of the supplied 96-well plate, mixed and is then added to the sample port of the cassette. Generally, the reaction is complete in 10-15 minutes. It is very important to note that the relative stoichiometry between the biotinylated antigen, biotinylated rabbit IgG added, and the streptavidin gold is critical for assay optimization. The appropriate concentration of biotinylated antigen to use with strips is dependent upon the purity and sequence and a standard curve can be used to determine the relative ratio (generally between 1ng-100ng per test). A positive control line (biotin-rabbit IgG) antibody will bind to the goat anti rabbit (GAR) line on the test to ensure the assay is running appropriately.
Attogene’s Human IgG/IgM universal lateral flow assay kit is a ready-to-use, universal test strip, which is based on the lateral flow technology that uses gold particles containing streptavidin to conveniently capture biotinylated antigens. The device is designed to easily develop qualitative or quantitative rapid test systems for detection of anti-human IgG and IgM antibody that react to the any antigen that can be biotinylated (i.e. viral antigen, autoimmune antigen) and is easily customizable providing every laboratory with the possibility to perform assay feasibility.
Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate as buffer.
How to use: 1. Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Salmonella typhi CMCC (B) 50071
good
Cloudy broth
2
Salmonella typhimurium CMCC (B) 50115
good
Cloudy broth
3
Paratyphoid Salmonella CMCC (B) 50093
good
Cloudy broth
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle; 225ml*10bag/box
Reference: 1.GB/T4789.4-2003 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms 2.GB/T4789.28-2003 People’s Republic of China national standards of food hygiene microbiological examination Staining, media and reagents 3.SN0170-92 People’s Republic of China Import and Export Commodity Inspection industry standard Salmonella food for export (including Arizona bacteria) test method 4.GB/T4789.4-2008 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms 5.GB 4789.4-2010 national standards for food safety standards of food hygiene inspection and microbiological testing of salmonella People’s Republic of China 6.GB 13091-91 People’s Republic of China National Standard Test Method for Salmonella in feed 7.GB 4789.40-2010 national standards for food safety standards of food hygiene inspection microorganism People’s Republic of China E.sakazakii test 8. ISO 6579-2002 Microbiology of food and animal feeding stuffs —– Horizontal method for the detection of Salmonella spp. 9. ISO22964-2006 Milk and milk products —– Detection of Enterobacter sakazakii 10. ISO6785-2001 Milk and milk products —– Detection of Salmonella spp
