Customize Consent Preferences

We use cookies to help you navigate efficiently and perform certain functions. You will find detailed information about all cookies under each consent category below.

The cookies that are categorized as "Necessary" are stored on your browser as they are essential for enabling the basic functionalities of the site. ... 

Always Active

Necessary cookies are required to enable the basic features of this site, such as providing secure log-in or adjusting your consent preferences. These cookies do not store any personally identifiable data.

No cookies to display.

Functional cookies help perform certain functionalities like sharing the content of the website on social media platforms, collecting feedback, and other third-party features.

No cookies to display.

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics such as the number of visitors, bounce rate, traffic source, etc.

No cookies to display.

Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors.

No cookies to display.

Advertisement cookies are used to provide visitors with customized advertisements based on the pages you visited previously and to analyze the effectiveness of the ad campaigns.

No cookies to display.

Universal Lateral Flow Assay Kit (Strep-Gold)

Facebook
X
Pinterest
Email

50 Lateral Flow Dipsticks (4.5mm)
10 mL Sample assay running buffer
96 well plate
Controls

Detail

Description

Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of  DNA and RNA products.

Formats (strep gold conjugate pad):

Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification.  Test line: anti-FITC/FAM, Control Line: Biotin

Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FAM and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.

Inquire about custom configurations: sales@attogene.com

Kit Components 

  • 50 -4.5mm Lateral Flow Dipsticks
  • 10 mL Sample assay running buffer

Features & Benefits

  • Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
  • No need to stripe capture antibodies
  • No expensive equipment required
  • Cost-effective way to screen for further downstream lateral flow assay development.

Other Products

HAM005 Peptone Bacteriological

Introduction

Usages:
Peptone obtained by enzymatic digestion of selected animal tissues, widely used in the preparation of microbiological culture media.

Technical specification:
Total nitrogen(TN)—————————≥14.5%
Amino nitrogen(AN) ————————≥2.0%
Moisture—————————————≤5.0%
Ash———————————————≤5.0%
Clˉ——————————————–≤3.0%
PH(2% solution)——————————5-7

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.

Specifications: 500g/bottle; 10kg /bag

D2141 HiPure DNA Clean Up Kit

Introduction

HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.

Details

Specifications

FeaturesSpecifications
Main FunctionsRecover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
ApplicationsPCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeCrude DNA, enzymatic reaction solution
Sample amount<150μl
Recovery80%
Elution volume≥15μl
Time per run≤10 minutes
Liquid carrying volume per column800µl
Binding yield of column20µg


Principle

The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

  • High recovery efficiency – up to 80% DNA recovery
  • Fast – isolation can be completed in 10 minutes by column gel method
  • Low elution volume – elution volume can be as low as 10μl
  • Efficient – efficient removal of various inhibitory factors

Kit Contents

ContentsD214102D214103
Purification Times100 Preps250 Preps
Buffer GWP30 ml60 ml
Buffer DW220 ml50 ml
Elution Buffer30 ml60 ml
HiPure DNA Mini Columns I50250
2 ml Collection Tubes50250

Storage and Stability

HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Milk Bacterial DNA Isolation Kit


Overview

  • Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
  • Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
  • Can process challenging samples such as mastitic milk
  • Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
  • Fast and efficient spin-column format

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​

Details

Supporting Data

Figure 1 / 3

Previous

Milk Bacterial DNA Isolation Kit Figure 1
Milk Bacterial DNA Isolation Kit Figure 1
Milk Bacterial DNA Isolation Kit Figure 2
Milk Bacterial DNA Isolation Kit Figure 3

Next

Click for expanded view

Kit Specifications
Maximum Milk Input1 mL
Time to Complete 10 Purifications1 hour
DNA Yield*500 ng to 8 μg
Bacteria Species ProcessedGram positive and Gram negative
Minimum Detection Limit10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

ComponentCat. 21550 (50 preps)
Resuspension Solution A6 mL
Buffer SK60 mL
Wash Solution A18 mL
Elution Buffer B15 mL
Proteinase K12 mg
Lysozyme (powder)120 mg
Spin Columns50
Collection Tubes50
Elution Tubes (1.7 mL)50
Product Insert1