Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. Test line: anti-FITC/FAM, Control Line: Biotin
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FAM and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.
Inquire about custom configurations: sales@attogene.com
Kit Components
50 -4.5mm Lateral Flow Dipsticks
10 mL Sample assay running buffer
Features & Benefits
Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
No need to stripe capture antibodies
No expensive equipment required
Cost-effective way to screen for further downstream lateral flow assay development.
Other Products
P1156 HiPure Plasmid EF Maxi Kit
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Product Info
Introduction
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications
Cell transfection, animal injection, etc.
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid vector
Sample amount
High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium
Yield
200-1500μg
Elution volume
≥0.7ml
Time per run
≤60 minutes
Liquid carrying volume per column
20ml
Binding yield of column
1mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 1.5mg plasmid can be binded in one column
Ultra low endotoxin – content of endotoxin is less than 0.1 EU/μg, which can be directly used for cell transfection and animal injection, etc.
Kit Contents
Contents
P115602
P115603
Purification Times
10 Preps
50 Preps
RNase A
30 mg
150 mg
Buffer P1
100 ml
500 ml
Buffer P2
100 ml
500 ml
Buffer LN3
50 ml
250 ml
Buffer PW1
33 ml
180 ml
Buffer PW2
20 ml
100 ml
Elution Buffer
15 ml
120 ml
Buffer CL
33 ml
180 ml
HiPure DNA Maxi Columns C
10
50
Lysate Clear Midi Syringe
10
50
50 ml Collection Tubes C
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Floor Type High Speed & Low Speed Universal Refrigerated Centrifuge
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Product Info
GL20 Technical Parameter:
Max. Speed
21000rpm
Max. RCF
30910×g
Max. Capacity
4×800ml
Time Range
1~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 60
Temperature
-20℃~40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
680×540×830mm
Net Weight(Kg)
170KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for GL20
Order No
Rotor
Rotor Max Speed(r/min)
Volume(ml)
RCF(×g)
G20-1
Swing Rotor
4000
4×800ml
3450
G20-2
Microplate rotor
4000
4×4×96 well
2940
G20-3
Microplate rotor
4000
2×4×96 well
3210
G20-4
Microplate rotor
4000
2×3×48 well
2300
G20-5
Swing rotor
4000
4×40×7ml vacuum tube
3270
G20-6
4000
4×40×5ml vacuum tube
2890
G20-7
4000
4×28×10ml vacuum tube
3130
G20-8
Swing Rotor
4000
Fat bottle
3830
G20-9
Fixed Rotor
16000
4×8PCR
15760
G20-10
Fixed Rotor
15000
6×8PCR
21420
G20-11
Fixed Rotor
16000
8×8PCR
17480
G20-12
Fixed Rotor
15000
12×8PCR
22930
G20-13
Fixed Rotor
15000
40×0.5ml
22920
G20-14
Fixed Rotor
21000
12×1.5ml
30910
G20-15
Fixed Rotor
16000
24×1.5ml
23440
G20-16
Fixed Rotor
14000
30×1.5ml
20800
G20-17
Fixed Rotor
13000
48×1.5ml
17930
G20-18
Fixed Rotor
16000
16×5ml
22020
G20-19
Fixed Rotor
16000
6×10ml
21500
G20-20
Fixed Rotor
15000
12×10ml
22680
G20-21
Fixed Rotor
13000
16×10ml
19490
G20-22
Fixed Rotor
13000
8×15ml
17790
G20-23
Fixed Rotor
11000
12×15ml
14330
G20-24
Fixed Rotor
5000
24×15ml
3500
G20-25
Fixed Rotor
5000
30×15ml
3830
G20-26
Fixed Rotor
14000
6×30ml
19060
G20-27
Fixed Rotor
13000
6×50ml(conical)
18840
G20-28
Fixed Rotor
13000
6×50ml(round)
18730
G20-29
Fixed Rotor
5000
12×50ml
3860
G20-30
Fixed Rotor
4000
24×50ml
2970
G20-31
Fixed Rotor
13000
4×85ml
18940
G20-32
Fixed Rotor
12000
4×100ml
14850
G20-33
Fixed Rotor
10000
6×100ml
11380
G20-34
Fixed Rotor
4000
12×100ml
2970
G20-35
Fixed Rotor
12000
24 capillaries
15800
G20-36
Swing Rotor
15000
4×5ml
19920
G20-37
Vertical rotor
16000
16×5ml
16450
G20-38
Vertical rotor
14000
8×30ml
19750
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Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and high signal intensity when compared with Brand A. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Features
High sensitivity and signal intensity
With ROX reference dye
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles. -20°C for 12 months
Document
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and high signal intensity when compared with Brand A. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.