Overview
- Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
- No phenol extractions
- Very sensitive and linear without the need for carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Isolate inhibitor-free urinary RNA
- Slurry/Spin column and Slurry/96-well format available
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating total RNA from urine for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Urine Total RNA Purification Maxi Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Details
Supporting Data
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| Kit Specifications | |
| Volume of Urine Processed | 5 – 10 mL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | < 30 minutes |
| Average Yield | Variable depending on specimen |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable until the expiration date specified on the label.
| Component | Cat. Dx29600 (50 preps) |
|---|---|
| RNA Lysis Solution | 3 x 90 mL |
| RNA Wash Solution | 24 mL |
| RNA Elution Solution | 6 mL |
| Mini Filter Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
