Vitamin B12, also called cobalamin, is a water-soluble vitamin that is involved in the metabolism of every cell of the human body: it is a cofactor in DNA synthesis, and in both fatty acid and amino acid metabolism. It is particularly important in the normal functioning of the nervous system via its role in the synthesis of myelin, and in the maturation of developing red blood cells in the bone marrow.
This kit is a product based on indirect-competitive ELISA, which is fast, easy, accurate and sensitive compared with common instrumental analysis.
Detection limit of Vitamin B12 acid in liquid samples
Other Products
HCM095 EC-MUG Medium
Product Info
Document
Product Info
Introduction
Intended Use
For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.
Principle and Interpretation
MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecalStreptococci.
Formulation
Ingredients
/liter
Trypticase or tryptose
20 g
Bile salts No. 3
1.5 g
Lactose
5 g
K2HPO4
4 g
KH2PO4
1.5 g
NaCl
5 g
4-methylumbelliferyl-β-D-glucuronide (MUG)
50mg
pH6.9±0.2 at 25°C
Preparation
Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until
completely dissolved, sterilize at 115℃ for 20min, cool to room temperature and set aside.
Quality Control
The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:
Quality control strains
Growth
Escherichia coli ATCC25922
Blue-white fluorescence is produced under 366nm UV light
Salmonella typhimurium ATCC14028
No fluorescence under 366nm UV light
Enterococcus faecalis ATCC29212
Clear, no fluorescence
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Isocitric Acid
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
1 to 80 µg of D-isocitric acid per assay
Limit of Detection:
0.35 mg/L
Reaction Time (min):
~ 3 min
Application examples:
Fruit juices, fruit products, soft drinks and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by EN, NEN, NF, DIN, GOST, IFU and AIJN
The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
