Vitamin B12 ELISA Kit

Highly Sensitive, rapid, robust screening kit for Vitamin B12

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Description

Vitamin B12, also called cobalamin, is a water-soluble vitamin that is involved in the metabolism of every cell of the human body: it is a cofactor in DNA synthesis, and in both fatty acid and amino acid metabolism. It is particularly important in the normal functioning of the nervous system via its role in the synthesis of myelin, and in the maturation of developing red blood cells in the bone marrow.

This kit is a product based on indirect-competitive ELISA, which is fast, easy, accurate and sensitive compared with common instrumental analysis.

Detection limit of Vitamin B12 acid in liquid samples

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Cells and Tissue DNA Isolation Kits

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Fast, reproducible and easy processing of samples
High yield and high quality genomic DNA with no RNA or protein contamination
DNA ready for any application including PCR, qPCR, genotyping and more

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit (Spin Column)

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit (Micro)

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 9

Figure 1. Isolation of High Quality Genomic DNA from Tissue

Figure 2. Isolation of High Quality Genomic DNA from 0.1 up to 3 million mammalian cells

Figure 3. Yield of Genomic DNA from 0.1 x 10^6 up to 3 x 10^6 mammalian cells

Figure 4. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 5. Resolution of DNA isolated from Animal Tissue (beef) and HeLa Cells

Figure 6. Comparison of A260/280 and A260/230 ratios

Figure 7. High Quality DNA confirmed by Real-time PCR

Figure 8. DNA was Isolated from 1 x 10^6 HeLa Cells

Figure 9. High Quality DNA was Confirmed by Real-Time PCR

Kit Specifications
Maximum Input	20 mg of animal tissue Up to 150 μL of viral suspension 3 x 10⁶ cells
Column Binding Capacity	> 50 μg
Average Yield	8 μg (from 1 x 10⁶ HeLa Cells) 10 μg (from 10 mg kidney)
Elution Volume	50 – 200 μL
Analyte Purified	Genomic DNA, mitochondrial DNA, viral DNA
Format	Spin Column
Time to Complete 10 Purifications	30 min (cells) and 90 min (tissue)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 53100 (50 preps)	Cat. 57300 (50 preps)	Cat. 59100 (50 preps)	Cat. 62500 (192 preps)
Lysis Buffer B	20 mL	20 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	18 mL	18 mL	55 mL
Wash Solution A	18 mL	18 mL	–	–
Elution Buffer B	30 mL	8 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K	1.2 mL	1.2 mL	1.2 mL	–
Proteinase K in Sotrage Buffer	–	–	–	4 mL
Magnetic Bead Suspension	–	–	2 x 1.1 mL	8.5 mL
96-Well Plate	–	–	–	2
Spin Columns	50	–	–	–
Micro Spin Columns	–	50	–	–
Collection Tubes	50	50	–	–
Elution Tubes (1.7 mL)	50	50	50	–
96-Well Elution Plate	–	–	–	2
Adhesive Tape	–	–	–	2
Product Insert	1	1	1	1

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Blood DNA Isolation Kits

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Overview

High yield and high purity DNA ready for any application
Available in a variety of formats to properly suit your needs
Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 19

Figure 1. The difference of Ct values obtained from a Taqman qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants

Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit.

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue)

Figure 18. DNA Isolated from Blood Preserved in EDTA Anticoagulants

Figure 19. Detection of GAPDH From Blood Samples Preserved in EDTA Anticoagulants

Kit Specifications
Maximum Blood Input	200 μL
Column Binding Capacity	> 50 μL
Average Yield (200 μL of blood)	2-8 μg*
Time to Complete 96 Purifications	45 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1

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TG16 Table Top High Speed Centrifuge

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Max. Speed	16000rpm
Max. RCF	20600×g
Max. Capacity	6×100ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (L x W x Hmm)	513×370×320mm
Net Weight/Gross weight(Kg)	48KG/55kg
Certificates	CE,ISO & Calibration report are available

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
G16-1	Angle rotor	14000	4×8PCR	12070
G16-2	Angle rotor	13000	6×8PCR	16080
G16-3	Angle rotor	14000	8×8PCR	13390
G16-4	Angle rotor	13000	12×8PCR	17220
G16-5	Angle Rotor	15000	30×0.5ml	18510
G16-6	Angle Rotor	14000	40×0.5ml	19970
G16-7	Angle Rotor	16000	12×1.5/2ml	17940
G16-8	Angle Rotor	15000	24×1.5/2ml	20600
G16-9	Angle Rotor	13500	30×1.5/2ml	19340
G16-10	Angle Rotor	11000	48×1.5/2ml	12840
G16-11	Angle Rotor	15000	16×5ml	19350
G16-12	Angle Rotor	14000	12×7ml	16370
G16-13	Angle Rotor	14000	6×10ml	16460
G16-14	Angle Rotor	13000	12×10ml	14510
G16-15	Angle Rotor	10000	12×15ml	11840
G16-16	Angle Rotor	5000	24×15ml	3080
G16-17	Angle Rotor	5000	30×15ml	3830
G16-18	Angle Rotor	12000	8×20ml	14510
G16-19	Angle Rotor	12000	6×30ml	14000
G16-20	Angle Rotor	11000	6×50ml	13480
G16-21	Angle Rotor	12000	6×70ml	10810
G16-22.1	Angle Rotor	12000	4x100ml	14850
G16-22	Angle Rotor	8000	6×100ml	7280
G16-23	Angle Rotor	12000	24 pieces capillary vessel	15800
G16-24	Microplate rotor	4000	2×3×48 Well	2300

Vitamin B12 ELISA Kit

Detail

Description

Other Products

Cells and Tissue DNA Isolation Kits

Overview

Details

Supporting Data

Blood DNA Isolation Kits

Overview

Details

Supporting Data

TG16 Table Top High Speed Centrifuge

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