Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 4 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.
Detail
Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 4 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom. ANSI/SBS footprint on top to accept all common microplates.
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable inconsistencies
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
Eliminates PCR inhibitors including humic acids
High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
96-Well and Magnetic Bead System formats also available
These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.
Stool DNA Isolation Kit (Spin Column)
Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation 96-Well Kit (High Throughput)
Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation Kit (Magnetic Bead System)
Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).
Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.
Free Download
Extracting Biological Insights from Stool
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Frozen, fresh or preserved stool
Format
Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume
50 μL
Time to Complete 10 Purifications
30 minutes
Applications
PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from tissue using economic salt out method
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Advantages
Safe – without phenol chloroform extraction
Environment friendly – the reagents used are safe, non-toxic and without pollution
High molecular weight – the molecular weight of genomic DNA is about 50-150kb
High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
Unlimited sample size – solution type operation, sample volume can be adjusted at will
Cost performance – the most economical nucleic acid extraction technology
Kit Contents
Contents
D331201
D331202
D331203
Purification Times
1 g
5 g
50 g
Proteinase K
330 µl
1.8 ml
18 ml
Buffer WTL
33 ml
160 ml
2 x 800 ml
Buffer PPS
12 ml
55 ml
500 ml
RNase Solution
330 µl
1.8 ml
18 ml
Buffer TE
12 ml
60 ml
200 ml
Storage and Stability
RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
