X96 Dual Ring Magnetic Plate 96-well with Integrated Cushion Base
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Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL (from PCR plates), larger outer magnet handles up to 2 mL deep well assays
Detail
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL (from PCR plates), larger outer magnet handles up to 2 mL deep well assays
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom, SBS/ SLAS fit on top to accept any microplate
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable differences
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation total RNA and DNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
Advantages
Post digestion sorting – higher DNA and RNA yields
Economy – the price is much lower than imported reagents
Kit Contents
Contents
IVD3026
Purification Times
200 Preps
MagBind Particles
9.0 ml
Proteinase K
180 mg
Protease Dissolve Buffer
10 ml
Buffer DPS
150 ml
Buffer FRL
40 ml
Buffer ATL
40 ml
Buffer AL
80 ml
Buffer BXW1*
110 ml
RNase Free Water
30 ml
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications
RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Cells: ≤1 x 107 Animal tissue: 1-20 mgPlant leaves: 50-150 mg Yeast cells: 5 x 106
Yield
2-100μg
Elution volume
≥50μl
Time per run
≤15 minutes（1-24 samples）
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 20 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
R401102
R401103
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
RTL Lysis Buffer
50 ml
200 ml
RNA Binding Buffer
15 ml
75 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;
2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;
3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of the centrifugal process, ensuring the safe operation of the instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code
Host Parameters
Product model
Mini-3
Input power supply
DC24V/2.75A
Input power
55W
Motor/Drive Method
DC24V brushless DC variable frequency motor
Display method
High brightness LCD screen
Effective centrifugal time
1-99min.1-59sec
speed
300~3000±15rpm
Speed step increase
10rpm
Maximum capacity
2 *96 well PCR plates/ELISA plates
MAX. RCF
608 xg
Misoperation/alarm failure
Sound prompt+display code
weight
3.9Kg
Noise
60dB (A)
Fastest acceleration time
<6s
Fastest deceleration time
<5s
Permissible ambient temperature/relative temperature
+5-40°C/80%
Document
The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .
