A device that uses the principle of high-frequency oscillation to generate heat through the friction of molecules in PVC hoses, thereby melting the PVC hose and achieving the sealing effect. This device is also referred to as a pipe sealing machine in some places.
| Heat Sealing Time: | 0.5~2s | Heat Sealing Tube Outer Diameter: | 2~6mm | ||||||
| Indicator Light Colors: | Red/Blue | RF Frequency: | 40.68MHz | ||||||
| Grounding Protection: | Class l | Spacing Adjustment | 65,70,75,80,85,90,95,100mm | ||||||
| Power Consumption: | 250W during operation,10Win standby | Power Supply: | 100V~120V/220V~240V AC | ||||||
| Weight: | 4.5kg | Dimensions: | 336×65×173mm | ||||||
| Operating Temperature: | 0~40℃ | Storage Temperature: | -20~70℃ | ||||||
This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.
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| Kit Specifications | |
| Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 250 mg |
| Time to Complete 96 Purifications | 50 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 26560 (192 preps) |
|---|---|
| Lysis Buffer G | 2 x 100 mL |
| Lysis Additive A | 25 mL |
| Binding Buffer I | 25 mL |
| OSR Solution | 12 mL |
| Wash Solution A | 2 x 38 mL |
| Elution Buffer B | 30 mL |
| Bead Tubes | 200 |
| HAR Plate | 2 |
| 96-Well Filter Plate | 2 |
| Adhesive Tape | 4 |
| 96-Well Collection Plate | 4 |
| 96-Well Elution Plate | 2 |
| Product Insert | 1 |
Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.
Listeria monocytogenes TaqMan PCR Kit, 100 reactions
Listeria monocytogenes TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM30450 (100 preps) | Cat. TM30410 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Listeria monocytogenes Primer & Probe Mix | 280 μL | 280 μL |
| Listeria monocytogenes Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
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Product Description
High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Product parameters:
| Reagent component (WLD8201-ES,2Tubes/box) | ||
| Component | Specification | Quantity |
| LD-1 | 800μL＝0.8mL | 1 Tube |
| LD-2 | 200μL＝1.2mL | 1 Tube |
Product features and advantages:
| Product features | |
| Simplicity | The operation is simple, and the DNA template for amplification can be obtained in just one step (conventional 5min) |
| Security | Non-toxic environmental protection, room temperature transport and storage |
| Universality | The lysate can be used for isothermal nucleic acid amplification |
Product application:
It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.
Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.
| Sample processing type | |
| Liquid sample | Samples of plants or tissues |
| Serum, blood, saliva, ascites and other samples, swab leach solution | Brain, muscle, viscera, leaves, fruit and other samples, take liquid supernatant |
