1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system.
Detail
Description
1X MOPS-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ Bis-Tris Precast Gel or polyacrylamide gels in Bis-Tris buffer system. It is convenient and universal for electrophoresis in Bis-Tris buffer system.
Features
Reliable: Rigorous quality control for reproducible separation of protein electrophoresis.
Convenient: Premeasured pouches make 1 liter of 1X buffer solution; No pH adjustment is necessary.
Fast: Dissolving in minutes and then ready to use.
Stable: Powder packaging suitable for long-term storage.
Contents
50mM Tris Base, 50mM MOPS, 0.1% SDS, 1mM EDTA
Applications
Running buffer for sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) in Bis-Tris buffer system
Storage
Room temperature for 24 months
Other Products
IVD5116 HiPure FFPE DNA/RNA Kit
Product Info
Document
Product Info
Introduction
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Details
Specifications
Features
Specifications
Main FunctionsC
Co-isolation DNA and RNA from a single FFPE tissue sample
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area.
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.
Advantages
1.Use non-toxic dewaxing solution without contact with xylene 2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)
Kit Contents
Contents
IVD5116
Purification Times
50 Preps
HiPure DNA Micro Column
50
HiPure RNA Mini Column I
50
2ml Collection Tubes
150
Proteinase K
50 mg
Protease Dissolve Buffer
5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer ATL
15 ml
Buffer RLC
15 ml
Buffer AL
15 ml
Buffer VHB
44 ml
Buffer RW2
25 ml
RNase Free Water
10 ml
Buffer AE
10 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Propargyl-PEG18-alcohol is a crosslinker that can react with azide compounds under the catalyzation of copper to form stable triazole linkage. The hydrophilic PEG units increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG18-alcohol is a crosslinker that can react with azide compounds under the catalyzation of copper to form stable triazole linkage. The hydrophilic PEG units increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Document
Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
