[CD4000] SMOChem™ dTTP Solution – Sodium Salt (100 mM), 25ml

Description

FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Features:

• Excellent for premix with DNA samples
• Sensitivity: 0.14 ng (DNA)
• A safer alternative to EtBr
• Compatibility: suitable to blue or UV light
• Increased cloning efficiency (blue light)

Composition 

FluoroDye™ DNA Fluorescent Loading Dye is stored in 6X concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.

Storage

Protected from light
 -20°C for 24 months

FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

OverView

These DNA polymerases are ideal for Isothermal Amplification

IsoPol® BST⁺ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST⁺ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Key features

• IsoPol® BST⁺ lacks 5’-3’- and 3’-5’-exonuclease activity.
• Inactivation – IsoPol® BST⁺ is completely inactivated after one minute incubation at 95°C
• Enhanced strand displacement and processivity.
• Increased salt tolerance.

IsoPol® BST⁺ Glycerol FREE 

Is the popular choice f or isothermal applications such as LAMP and RT-LAMP at point-of-care diagnostics for its superior amplification performance and robustness. The enzyme is offered in a 1X storage buffer at a concentration < 300 U/µl

IsoPol® BST⁺ High Concentration Glycerol FREE

(IsoPol® BST⁺ HC Glycerol FREE) is used to ease the product development processes in sequencing technologies, solid phase amplification and several isothermal technologies. Same performance as IsoPol® BST⁺ (i.e., high processivity, relative SDA, and relative activity) with the only difference that it is offered in a 2X storage buffer at a concentration > 500 U/

Key features

• High concentration
• High purity
• Lyophilisation and automation friendly

Figures

Properties

These DNA polymerases are ideal for Isothermal Amplification

IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Overview

• Isolate genomic DNA from either exfoliated cells or bacteria found in urine
• Isolate and detect genomic DNA from as little as 1 mL of urine and up to 50 mL urine
• High quality DNA for sensitive applications
• Rapid processing time

Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.

Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.

Details

Supporting Data

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Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.