[PM2800] ExcelBand™ 3-color Extra Range Protein Marker (10-310 kDa), 250 μl x 2
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The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Detail
Description
The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Three reference bands — 310 kDa (red), 75 kDa (red) and 25 kDa (green)
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, PM2800 3-color Extra Range Protein Marker resolves 13 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months -20°C for long term storage
Other Products
endo-BCN-PEG2-PFP ester
Product Info
Document
Product Info
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from whole blood using economic salt out method
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Advantages
Safe – without phenol chloroform extraction
Environment friendly – the reagents used are safe, non-toxic and without pollution
High molecular weight – the molecular weight of genomic DNA is about 50-150kb
High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
Unlimited sample size – solution type operation, sample volume can be adjusted at will
Cost performance – the most economical nucleic acid extraction technology
Kit Contents
Contents
D331101
D331102
D331103
Purification Volumes
50 ml
300 ml
1000 ml
10 x Buffer RBC
20 ml
100 ml
3 x 100 ml
Buffer WTL
60 ml
350 ml
1000 ml
Buffer PPS
20 ml
120 ml
350 ml
RNase Solution
300 µl
1.8 ml
6 ml
Buffer TE
10 ml
30 ml
120 ml
Storage and Stability
RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.
About
Our 384-well plate has a rigid, fully-skirted frame which remains stable during thermal cycling. The rigidity of the plate lends itself to automated and high-throughput PCR environments where robotic handling maybe in use.
The 2-component design reduces the risk of evaporation due to a poor sealing, commonly seen with 1-component PCR plates. 100% virgin medical grade polypropylene is used for the 384 wells, with a maximum capacity of 45 µL Certified free from DNase, RNase, nucleases and human gDNA.
