DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Total Nucleic Acid Preservation Tubes Dx are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The Preservative prevents the growth of Gram-negative and Gram- positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months. This kit is ideal for collecting and preserving nasal and oral swab samples for in vitro diagnostic use for medical purposes. This kit can be used to preserve viral RNA, including SARs-CoV-2 RNA for detection of COVID-19.
Norgen’s Total Nucleic Acid Preservation Tubes Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.
Norgen’s Total Nucleic Acid Preservation Tubes Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate these devices in conjunction with a downstream in vitro diagnostic assay.
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| Kit Specifications | |
| Stability of DNA at Room Temperature | 4 months |
| Stability of RNA at Room Temperature | 2 months |
Shelf Life and HandlingThe tubes should be kept tightly sealed and stored at room temperature for up to 1 year without any reduction in performance (The collection due date is written on the device label and the kit box label).Once collected, RNA is stable for up to 2 months and DNA is stable for up to 4 months when the tubes are kept tightly sealed and stored at room temperature.
| Kit Components | Cat. Dx69200 |
|---|---|
| Total Nucleic Acid Preservation Tubes Dx | 50 |
| Product Insert | 1 |
GL12 Technical Parameter：
| Max. Speed | 12000rpm |
| Max. RCF | 22650×g |
| Max. Capacity | 4×1000ml |
| Timer | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 60 |
| Temperature Range | -20~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (L x W x Hmm) | 780×675×830mm |
| Net Weight(Kg) | 260KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL12
| Order No. | Rotor No. | Max Speed (rpm) | Max Volume(ml) | Max.RCF（×g） |
| L12-1 | Angle Rotor | 12000 | 6×250/300ml | 22650 |
| L12-2 | Angle Rotor | 8000 | 6×500ml | 11740 |
| L12-3 | Swing Rotor | 4000 | 4×1000ml | 4060 |
| L12-4 | Microplate Rotor | 4000 | 4×4×96well | 2940 |
| L12-5 | 4000 | 2×4×96well | 2490 | |
| L12-6 | Angle Rotor | 10000 | 4×500ml | 16110 |
Features
1. Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.
2. Brushless frequency motor for model GL12 which in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. Model GL12 with the digital display which indicates the speed、time and temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. There are high speed rotors and low speed rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
Model:
GL12
Brand:
Yingtai
Code:
8421199090
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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.
Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.
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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.
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