DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow
Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres can irreversibly bind biotin.
Europium dyed polystyrene beads conjugated to streptavidin
250ul of a 1mg/ml stock ready to use for Lateral Flow
50mL Lateral FLow Running Buffer designed for Streptavidin Europium Chelate Microspheres
Inquire for Larger Volumes: [email protected]
Excitation max: 365nm
Emission max: 610nm
Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow
Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres can irreversibly bind biotin.
Norgen’s FBS Exosome Depletion Kits provides a quick and easy protocol for the depletion of bovine exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen’s kits allow for the processing of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for exosome depletion from FBS, as compared to the expensive current ready-to-use exosome-depleted media available on the market.
Background
Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of bovine exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.
FBS Exosome Depletion Kit (Slurry Format)
For FBS volumes ranging from 140 mL to 280 mL.
FBS Exosome Depletion Kit (Column Format)
For FBS volumes ranging from 120 mL to 240 mL.
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| Kit Specifications | |
| Sample Type | Fetal Bovine Serum |
| Sample Volume Range | Up to 140 mL (FBS Exosome Depletion Kit I (Slurry Format) Up to 280 mL (FBS Exosome Depletion Kit II (Slurry Format) |
| Depletion | Deplete exosome-sized vesicle |
| Bovine miRNA | No detectable bovine miRNA |
| Time to Complete 6 Purifications | 40 minutes |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 61100 (6 preps) | Cat. 61400 (12 preps) | Cat. 61200 (6 preps) | Cat. 61300 (12 preps) |
|---|---|---|---|---|
| ExoC Buffer | 2 x 1.5 mL | 8 mL | 2 x 1.5 mL | 8 mL |
| Slurry E | 12.5 mL | 2 x 12.5 mL | – | – |
| Maxi Spin Column | – | – | 6 | 12 |
| Product Insert | 1 | 1 | 1 | 1 |
Cell Culture Dish 150mm
Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation
Specifications: 35mm. 60mm.100mm.150mm
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation
Specifications: 35mm. 60mm.100mm.150mm
