[RI1001] RNAok™ RNase Inhibitor, 20 U/μl, 2000 U x 5

Overview

• Ensure the stability and viability of viruses, chlamydia, and mycoplasma.
• Manufactured based on CDC formula.
• Antimicrobic blend suppresses bacterial and fungal contamination.
• Supplied in bulk (1 Litre) to provide options for custom applications.
• Preserve swab samples for up to 48h when preserved at 2–8°C.

Norgen’s Viral Transport Medium is formulated to transport samples that are presumably infected with a virus. Viral Transport Medium is manufactured based on CDC formula and is offered in bulk to provide an option to readily assemble swab collection devices for sample collection.

Norgen’s Viral Transport Medium (VTM) is designed for safe and efficient transport of viral samples, including but not limited to those from nasal, throat, and other swabs. The VTM contains a balanced mix of Hank’s Balanced Salt Solution (HBSS), heat-inactivated fetal bovine serum (FBS), gentamicin sulfate, and amphotericin B to preserve the viability of viruses and prevent the growth of contaminating bacteria and fungi. The medium ensures the stability and viability of viruses, chlamydia, and mycoplasma for analysis using different techniques. In addition, the Viral Transport Medium eliminates the need to immediately process or freeze samples.

Details

Storage Conditions and Product Stability
The Viral Transport Medium can be stored at 2-8°C for up to 1 year from the date of manufacturing without any reduction in performance.

Overview

• Purification and enrichment of intact cell culture media exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• Versatile cell culture media input volumes
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents nor overnight incubation required
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

For sample input volumes ranging from 5 mL to 10 mL.

Cell Culture Media Exosome Purification and RNA Isolation Midi Kit

For sample input volumes ranging from 10 mL to 20 mL.

Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit

For sample input volumes ranging from 20 mL to 35 mL.

Details

Supporting Data

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Kit Specifications
Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	20 mL to 35 mL
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	40 to 45 minutes
Average Yields*	Variable depending on specimen

* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Cat. 60700 (50 preps)	Cat. 60800 (25 preps)	Cat. 60900 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	1.5 mL	1.5 mL	1.5 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	18 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Column	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Control  System:   

 5.4”LCD   Touch   Screen   Display

>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.

>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.

>Ithas  the  functions  of  cold  trap  precooling  and  vacuum  pump  preheating,and  the  time  can  be  set  by  the  userin  the  system. >Display  whether  the  current  running  status  is  normal  or  not  by  numerical  color.

>Built-in    saturated    vapor     pressure    &temperature    corresponding     relationship    table,dearly     displaying    the    corresponding     relationship.

o One-key operation,the operation is extremely simple,no special professional learning and training is required.

o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably. 

o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.

o Ambient    temperature    monitoring,the    device    automatically     reminds    the    alarm    if    the    ambient    temperature     is    too    high.

o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.

Drying chamber	· The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external   valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and  various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional)
Cold trap andcondensing coil	· The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation.
Sample shelves	· 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive  solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf.
Refrigerationsystem	· Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures.

Techinical parameter

Item No	ST7003	ST7006		ST9006
Ice condenser capacity	3kg	6kg		6kg
Ice condenser temperature	-70℃	-70℃		-90℃
Cold trap volume	5L	10L		10L
Freeze dried chamber	Bell shape (standard configuration)/Multi manifold type/T-shaped frame type
Sample shelf	φ200mm.Single layer area 0.03m2 with 3-5 layers available	●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona
Vacuum pump	Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filte	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment	Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents.
External valve	6pieces/optional	1-48 pieces/optional
Eutectic point detection system	Support/Optional
Data output analysis	Support/Optional
Powe	0.6kw	1.1kw		1.5kw
Size	420*545*455mm		510*570*500mm

Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006

Brand:
Yingtai