Description
The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.
Features
- High yield
- Thermostable, up to 50°C, during first strand synthesis
- High processivity, generating cDNA up to 8 kb
- Reduced RNase H ribonuclease activity
Application
- Generation of first strand cDNA from total RNA or mRNA.
- Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.
Storage
-20°C for 24 months
High yield
Thermostable, up to 50°C, during first strand synthesis
High sensitivity
Contents
| ComponentVolumeExcelRT™ Reverse Transcriptase (200 U/μl) 100 μl5X RT Buffer1 ml0.1 M DTT 500 μl |
Storage Buffer
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
5X RT buffer
250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl and 15 mM MgCl2
Storage
-20°C for 24 months
