Introduction
This product is suitable for rapid extraction of DNA from plant and fungal samples. The kit is based on silica gel column purification technology. The whole extraction process only takes 60-90 minutes. DNA can be used directly for PCR, quantitative PCR, southern Blot, test of virus DNA and so on.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50mg simple plant using 96 well bind plate |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount | Fresh / frozen plant samples: ≤50mgDried plant / seed samples: ≤15mg |
| Elution volume | ≥75μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
- High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, blot hybridization, etc.
- High throughput – 96 samples can be processed simultaneously by 96 well plate
- High permeability – using composite membrane technology, no hole plugging
Kit Contents
| Contents | D316702 | D316703 |
| Purification Times | 4 x 96 Preps | 20 x 96 Preps |
| RNase Solution | 5 ml | 22 ml |
| Elution Buffer | 120 ml | 500 ml |
| Buffer SPL | 200 ml | 2 x 500 ml |
| Buffer PS | 100 ml | 400 ml |
| Buffer GW1 | 220 ml | 5 x 220 ml |
| Buffer GW2 | 100 ml | 3 x 100 ml |
| 1.6ml Collection Plate | 4 | 20 |
| HiPure gDNA Plate | 4 | 20 |
| 2.2ml Collection Plate(DW Plate) | 8 | 40 |
| 0.8ml Collection Plate(DW Plate) | 4 | 20 |
| Sealing Film | 20 | 100 |
Storage and Stability
RNase solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Purchase Guide
| Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
| HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
