384-WELL SLIM PCR PLATE

Overview

• Detection kits for Bacillus cereus
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Bacillus cereus is a rod-shaped, gram-positive bacterium. Some strains of B. cereus cause foodpoisoning and other diseases such as keratitis. B. cereus grows at a wide range of temperature, from 4°C to 37°C. In fact, many foodborne illnesses caused by B. cereus are a consequence of improperly cooked or improperly stored food, including dairy products and meats. The majority of food poisoning by B. cereus could be divided into two types of symptoms – the emetic type and the diarrheal type, both of which are caused by the enterotoxin produced by the bacteria.

Bacillus cereus TaqMan PCR Kit,
100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Bacillus cereus TaqMan PCR Probe/Primer Set and Controls,
100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM36950 (100 preps)	Cat. TM36910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Bacillus cereus Primer & Probe Mix	280 μL	280 μL
Bacillus cereus Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Biotin is extensively used in combination with streptavidin in biological assays.  Our biotin detection kits have multiple applications in detecting biotin attachment and efficiency of conjugation during biotinylation processes.  These kits are perfect for understanding the efficiency of the reaction and free biotin in your solutions that can interfear with Streptavidin-biotin interactions.

Attogene offers three kits to detect and quantify biotin using three different technologies including Lateral Flow, ELISA and Chemical.  these kits are accurate and sensitive compared with common instrumental analysis.

Can be used to calculate the amount of biotin in liquid samples including biological samples including biotinylated antibody, free biotin in solution, foods, plasma and serum as needed.  Also, can be used to quantify the % biotinylation following standard biotinylation reactions.

EL2021_EL: ELISA Biotinylation Quantification Kit

EL2021_EZ:  TNBSA Biotinylation Quantification Kit

EL2021_LF: Lateral Flow kit Biotinylation Detection Kit

Highly Sensitive, rapid, robust screening kit for Biotin
Useful for detecting and quantifying biotin in biological samples
Adaptable for detecting biotin in food samples

Introduction

Usages:
For enumerating and enriching nonfastidious or fastidious bacteria.

Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance.

Formulation(per liter):
Pancreatic Digest of Casein 15g
Papaic Digest of Soybean 5g
Sodium chloride 5g
Agar 15g
Final pH 7.3±0.2

How to use:
1.Suspend 40g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g