2 Layer Cell Factory Double Wide Mouth

• For sizing and quantification of double strand DNA fragments.
• Composed of 5 bands from 10 kb to 48.5 kb.
• Premixed with 6X DNA loading buffer for direct gel loading.

• For sizing and quantification of double strand DNA fragments.
• Composed of 5 bands from 10 kb to 48.5 kb.
• Premixed with 6X DNA loading buffer for direct gel loading.

Product Description

Product Detail

Kit Storage and Term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal Nucleic Acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

 

Overview

• High yield and high purity DNA ready for any application
• Available in a variety of formats to properly suit your needs
• Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 19

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Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1