40 Layer Cell Factory Double Wide Mouth

Huayi Cell Factory is a robust and easy-to-use cell

culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.

40 Layer Cell Factory Double Wide Mouth

Huayi Cell Factory is a robust and easy-to-use cell

culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.

PRODUCT FEATURES

The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Surface TC treatment ensures efficient cell attachment
The product assembled with ultrasonic welded technology
Versatile port design facilitates both pouring and aseptic filling techniques
Gamma radiation sterilization
the cell culture surface area

of one 10-layer Cell Factory unit is equivalent to the area

of 36 T-175 flasks

Other Products

Urine Exosome and Free-Circulating RNA Isolation Kits

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Overview

Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
Versatile sample input ranges
Isolate all sizes of free-circulating RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
The purified exosomal RNA is free from any circulating RNA-binding proteins
No phenol extractions, Proteinase K treatment nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes are required
No precipitation reagents nor overnight incubation required
Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Details

Supporting Data

Figure 1 / 6

Figure 1. Urine Exosome and Free-Circulating RNA Isolation Mini Kit

Figure 2. Urine Exosome and Free-Circulating RNA Isolation Mini Kit

Figure 3. Urine Exosome and Free-Circulating RNA Isolation Midi Kit

Figure 4. Urine Exosome and Free-Circulating RNA Isolation Midi Kit

Figure 5. Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

Figure 6. Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

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Kit Specifications
Minimum Urine Input	2 mL
Maximum Urine Input	10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50 – 100 µL
Time to Complete 10 Purifications	35 – 40 minutes
Average Yields*	Variable depending on the specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component	Cat. 59200 (50 preps)	Cat. 59300 (25 preps)	Cat. 59400 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	2 x 20 mL	2 x 20 mL	2 x 20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	2 x 18 mL	18 mL	18 mL
Elution Solution A	2 x 6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	100	50	30
Collection Tubes	100	50	30
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

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022021P1 Nutrient Agar

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D6359D MagPure Forensic DNA Kit

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Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	Dark red
2	Aerogenes CMCC (B) 45103	Good	red
3	Staphylococcus aureus ATCC6538	Suppressed	—

Contents	D635901D	D635902D	D635903D
Purification Times	48	96	480
MagPure Particles N	1.1 ml	2.2 ml	11 ml
Buffer ATL	40 ml	80 ml	2 x 200 ml
Buffer BGL	40 ml	80 ml	2 x 200 ml
Buffer BST1	60 ml	120 ml	550 ml
Buffer GW2*	20 ml	25 ml	2 x 50 ml
Proteinase K	48 mg	110 mg	2 x 220 mg
Protease Dissolve Buffer	5 ml	10 ml	30 ml
DTT	235 mg	235 mg	3 x 235 mg
Elution Buffer	10 ml	10 ml	30 ml

Product	Contents and volume	D6359D-TL-06
Buffer BGL		80 ml
Buffer ATL		80 ml
Proteinase K		110 mg
Protease Dissolve Buffer		10 ml
DTT Powder		2 x 235 mg
Elution Buffer		5 ml
AS-Tip		12 pcs
2.0ml V bottom plate	Row 1/7：empty	6 plates
	Row 2/8：500μl Buffer BST1
	Row 3/9：500μl Buffer BST1
	Row 4/10：20μl MagPure Particles N 500μl Buffer GW2 Row 5/11：500μl Buffer GW2 (ethanol ~350ul)
	Row 6/12：60μl Elution Buffer

Product	Contents and volume	D6359D-F-96	D6359D-F-48
Buffer BGL		80 ml	40 ml
Buffer ATL		80 ml	40 ml
Proteinase K		110 mg	48 mg
Protease Dissolve Buffer		10 ml	5 ml
DTT Powder		235 mg	235 mg
Elution Buffer		5 ml	5 ml
96-Tip (AS)		1	1
Sample Plate 1	500μl Buffer BST1	1	1
Sample Plate 2	500μl Buffer BST1	1	1
Wash Plate 1	20μl MagPure Particles N500μl Buffer GW2	1	1
Wash Plate 2	500μl Buffer GW2	1	1
Elute Plate	60μl Elution Buffer	1	1

40 Layer Cell Factory Double Wide Mouth

Detail

Other Products

Urine Exosome and Free-Circulating RNA Isolation Kits

Overview

Details

Supporting Data

022021P1 Nutrient Agar

Introduction

D6359D MagPure Forensic DNA Kit

Introduction

Details

Kit Contents

Prefilled Kit Contents

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