endo-BCN-PEG2-amine is a heterobifunctional PEG linker containing a BCN and an amine group. The BCN group can react with azide-tagged molecules. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-amine is a heterobifunctional PEG linker containing a BCN and an amine group. The BCN group can react with azide-tagged molecules. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from <25 mg tissue, culture cells, FTA card
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissue or cultured cells
Sample amount	Animal tissue : <25mg, Cultured cells : <5 x 106
Elution volume	≥30μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
• High purity – can be used for downstream applications such as multiplex and quantitative PCR
• Fast – simplified process, extracting several samples in 20 minutes (after digestion)
• Safety – no phenol orchloroform extraction, no alcohol precipitation

Kit Contents

Contents	D312102	D312103
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	65 ml
Buffer DL	15 ml	65 ml
Buffer GW1	22 ml	110 ml
Buffer GW2	12 ml	50 ml
RNase A	10 mg	50 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml
HiPure gDNA Mini Columns	50	2 x 125
2 ml Collection Tubes	100	5 x 100

Storage and Stability

RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Compact and stylish designed, the BioShake iQ high-end thermoshaker lets you perform all your standard mixing runs with a minimum of adjustments.
The BioShake iQ offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.

Small. Light. Efficient. Quiet.

• For microplates, tubes and glass vials
• Fast & high-precision heating
• Temperature range: ambient to 99°C
• Precise mixing from 200 – 3,000 rpm
• Wide range of thermo adapters
• Stylish aluminium housing

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ADVANCED TECHNOLOGY

Save time.

By combining the mixing operation with the incubation phase, reaction process times and operator workload are reduced and efficiency of many procedures is increased, resulting in a higher throughput.

The first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.

GENTLE MIXING OF SAMPLES

By planar orbital motion

German designed and manufactured, BioShake iQ thermoshaker offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.

The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.

Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.

HOMOGENEOUS HEATING

Using large-area heating source in combination with digital sensor electronic

The BioShake iQ heats from ambient to 99°C in a short time, provides ease of use features, amazing comfort and maximum safety.

Within the BioShake iQ thinnest large-area heating elements and sensors are integrated. During the heat up time the special electronic control system guarantees the same temperature for all vessels with minimal deviations.
Temperatures are selectable from ambient temperature to 99°C in steps of 1.0°C. The nominal regulation accuracy is better than ±0.1°C with a uniformity of temperature distribution better than ±0.5°C at 45°C across the heating surface.

Exchangeable thermo adapter plates and thermal blocks enable a perfect adaptation to microplates, tubes or glass vials.

UNIVERSAL SPRING CLAMPS

Suitable for all microplates

In the corners eight spring clamps are inserted for easy fixation of skirted microplates and deep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.

For flexible and deformable PCR plates we recommend using of the related PCR adapter.

Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.

COMFORT

Simple intuitive operation and programming

The BioShake iQ is operated from the front face of the enclosure which includes touch buttons and a display. 

Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.

The short mix button allows short and fast mixing in between.

The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time, mixing frequency and temperature.

EXCHANGABLE THERMO ADAPTERS

For a wide range of applications

The BioShake iQ comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.

Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels. An excellent temperature uniformity and homogeneity is guaranteed for all samples.

UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY

First-class finished aluminium housing

Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.

Safety of the user and ease of use are clearly a priority.

MADE IN GERMANY

High-end instruments for daily routine

A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.

The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.

For microplates, tubes and glass vials
Fast & high-precision heating
Temperature range: ambient to 99°C
Precise mixing from 200 – 3,000 rpm
Wide range of thermo adapters
Stylish aluminium housing