Overview

• Detection kits for Listeria monocytogenes
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis.

Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.

Listeria monocytogenes TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Listeria monocytogenes TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM30450 (100 preps)	Cat. TM30410 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Listeria monocytogenes Primer & Probe Mix	280 μL	280 μL
Listeria monocytogenes Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate
Applications	Enzyme digestion, sequencing, PCR, labeling, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	1-1.5ml（x96）
Yield	1-15µg/1ml
Elution volume	≥70μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	70µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 60 minutes to complete the isolation
• High yield – up to 1mg plasmid can be binded in one column
• Economy – high cost performance

Kit Contents

Contents	P100601	P100602	P100603
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
RNase A	5 mg	20 mg	100 mg
Buffer P1	30 ml	120 ml	600 ml
Buffer P2	30 ml	120 ml	600 ml
Buffer P3	40 ml	180 ml	800 ml
Buffer PW1	100 ml	500 ml	2 x 1000 ml
Buffer PW2	50 ml	2 x 100 ml	4 x 200 ml
Elution Buffer	150 ml	60 ml	300 ml
Lysate Clear Plate	1	4	20
HiPure DNA Plate	1	4	20
1.6 ml Collection Plate	1	4	20
0.5ml Elute Plate	1	4	20

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Features	Sampleamount	Elutionvolume	Yield
HiPure Plasmid Mini Kit	P1001	Classic, Regular application	1-5ml LB	30-50μl	5–35µg/5ml
HiPure Plasmid Mini Kit II	P1002	Classic, Regular application	5-15ml LB	75-100μl	20-80µg/15ml
HiPure Plasmid Plus 96 Kit	P1006	Classic, Regular application	1-1.5ml LB	70-150μl	1-15μg/1ml
HiPure Fastfilter Plasmid Midi Kit	P1013	Fast	30-50ml LB	0.3-0.8ml	50–250µg/50ml
HiPure Fastfilter Plasmid Maxi Kit	P1014	Fast	100-200ml LB	0.5-1.5ml	0.4–1mg/200ml
Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit	P1156	Classic method, General for all kinds of vectors	100-200ml LB	≥0.7ml	200–1500µg
HiPure Plasmid EF Mini Kit	P1154	Recommend for low copy vector, Thoroughly remove RNA	5-15ml LB	≥60μl	10–80µg
HiPure Plasmid EF 96 Kit	P1157	Recommend for low copy vector, Thoroughly remove RNA	1-5ml LBx96	≥75μl	30µg
HiPure Plasmid EF Midi Kit	P1231	Recommend for High copy vector, Low elution volume, High concentration	25-50ml LB	≥100μl	10-500μg
HiPure Plasmid EF Mega Kit	P1116	Recommend for High copy vector(>3μg/ml)	500ml LB	≥2ml	1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Plasmid DNA Plus 96 Kit enables up to 96 minipreps to be performed simultaneously in less than 45 minutes on the Vacuum Manifold (Qiavac 96). This kit provides a fast, simple,and cost-effective plasmid DNA high-throughput method for routine molecular biology laboratory applications. HiPure Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

High Purity DNA Purification Kit

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12 Months DNA Purification Kit

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Nucleic Acid dna isolation kit

Product Description

Rapid Nucleic Acid Release Reagent (DNA) – Type II

High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids

Product parameters:

Reagent component  (WLD8201-ES,2Tubes/box)
Component	Specification	Quantity
LD-1	800μL＝0.8mL	1 Tube
LD-2	200μL＝1.2mL	1 Tube

 Product features and advantages:

Product features
Simplicity	The operation is simple, and the DNA template for amplification can be     obtained in just one step (conventional 5min)
Security	Non-toxic environmental protection, room temperature transport and storage
Universality	The lysate can be used for isothermal nucleic acid amplification

 Product application:

  It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.

  Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.

Sample processing type
Liquid sample	Samples of plants or tissues
Serum, blood, saliva, ascites and other samples,   swab leach solution	Brain, muscle, viscera, leaves, fruit and other   samples, take liquid supernatant