96-Well PCR Plate / 0.2 mL Strip Chiller Block

Description 

The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.

Application

• cDNA synthesis
• in vitro translation
• in vitro transcription
• One-step RT-PCR
• Separation and identification of specific ribonuclease activities

Storage Buffer

40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT,   0.1 mM EDTA, stabilizer and 50% (v/v) glycerol

Storage

-20°C for 24 months 

The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.

Overview

• Extract high quality & quantity total RNA including miRNA
• No phenol step required; isolate all RNA in one fraction
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Available in a variety of formats to suit your needs
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A₂₆₀/A₂₈₀ suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 106 cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 109 cells
Yeast	1 x 108 cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 106 cells)	15 μg
E. coli (1 x 109 cells)	50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen’s Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1

Introduction

Usages:
For monitoring and detection of surface of equipment and personnel hygiene.

Advantage:
1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life.
2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability.
3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation.
4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit..
5.This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.

Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.

Specifications: 55mm*10 plates / bag

55mm*10 plates / bag