α-Amylase SD Assay Kits

Overview

• Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive
• Bead tubes (provided) allow for effective mechanical homogenization
• Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more

These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.

Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)

This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.

Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)

Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.

Details

Supporting Data

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Kit Specifications
Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	All sizes
Maximum Amount of Starting Material: Fungi (Wet weight) Yeast or Gram-positive bacterial culture	50 mg 0.5 mL – 1 mL
Time to Complete 96 Purifications	40 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. 27300 (50 preps)	Cat. 27350 (192 preps)
Lysis Buffer L	30 mL	2 x 60 mL
Resuspension Solution A	20 mL	60 mL
Solution BX	28 mL	2 x 28 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Buffer B	15 mL	30 mL
Bead Tubes	50	200
Spin Columns	50	–
Collection Tubes	50	–
96-Well Plate	–	2
96-Well Collection Plate	–	2
Adhesive Tape	–	4
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1

Description 

The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program.

Features

• Reverse transcription at wide temperature range (42-60°C)
• High specificity 
• Suitable for fast cycle program
• With no ROX reference dye

Storage

Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)

Protect from light

-20°C for 12 months

The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, no ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step Master Mix features high specificity and is suitable for fast cycle program.

Overview

• Extract high quality & quantity total RNA including miRNA 
• No phenol step required; isolate all RNA in one fraction
• Genomic DNA Removal Column for efficient elimination of gDNA
• Bind & elute all RNA irrespective of size or GC content, without bias
• Efficiently extract small RNA irrespective of GC content
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Isolate from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step. 

Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more.  Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.  Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Column	Up to 50 μg RNA
Maximum Loading Volume Per Spin Column	650 μL
Size of RNA Purified	All sizes, including < 200 nt
Time to Complete 10 Purifications	25 minutes
Maximum Amount of Starting Material
Liver Tissue	20 mg
Heart Tissue	5 mg
Kidney Tissue	20 mg
Brain Tissue	25 mg
Lung Tissue	20 mg
Spleen Tissue	20 mg
Whole Blood	100 µL
Hela Cells	3 x 106
CHO	3 x 106
Yeast	1 x 108 cells
E.coli	1 x 109 CFU/mL
Bacteria	1 x 109 cells
Plasma/Serum	200 µL
Fungi	50 mg
Plant Tissue	50 mg
Viruses	≤100 µL of viral suspension
Average Yield
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48300 (50 preps)	Cat. 48400 (100 preps)
Buffer RL	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 6 mL
RNA Purification Columns	50	100
Genomic DNA Removal Columns	50	100
Collection Tubes	100	200
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1