The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.
Detail
K-FRUCHK
SKU: 700004286
50 assays per kit
Content:
50 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Fructan
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
4 to 80 µg of D-glucose, D-fructose or sucrose per assay
Limit of Detection:
1 g/100 g
Total Assay Time:
~ 30 min
Application examples:
Flours, plant materials (e.g. onion), food products and other materials
The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.
All reagents stable for > 12 months after preparation
Fructan kits are available only from Megazyme
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
Staphylococcus aureus Quantified Bacterial DNA Standard
Product Info
Document
Product Info
Overview
Quantified standard to be used as a positive control or PCR quantification standard
Vigorously quantified using multiple methods
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus (S. aureus) is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry. S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulase-positive, which forms the basis of traditional identification methodology.
Staphylococcus aureus Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Staphylococcus aureus.
Upon receipt, store Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Micronucleic acid extraction and purification, virus total nucleic acid extraction
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/F, 3 layers
Membrane aperture
0.7μm
Maximum binding yield of plasmid
10 μg
Maximum yield of alcohol mediated Binding
50 μg
Single liquid carrying capacity of column
700 μl
Minimum elution volume
15 μl
Withstand centrifugal force
12,000 x g
Centrifuge
Small high speed centrifuge (2ml)
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13011
HiPure DNA Micro Column I (3 x GF/F)with 2ml Collection Tubes
1000/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
AZtaq™ DNA Polymerase is a high-quality DNA polymerase, originating form Thermus aquaticus. Being highly thermostable, AZtaq is ideal for use in polymerase chain reaction (PCR) applications.
The enzyme catalyses the synthesis of a complementary DNA strand using a primed DNA or cDNA strand as template. It possesses 5’-3’ exonuclease activity while lacking 3’-5’ proofreading activity.
AZtaq is compatible with the use of dUTP, enabling highly efficient removal of carry-over contamination with Cod UNG.
Key Features
Excellent qPCR Performance
Compatible with dUTP
Thermostable
Applications
PCR/qPCR
Figures
Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. AZtaq is manufactured at our ISO 13485 certified facility in Norway.
Document
AZtaq™ DNA Polymerase is a high-quality DNA polymerase, originating form Thermus aquaticus. Being highly thermostable, AZtaq is ideal for use in polymerase chain reaction (PCR) applications.
