The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
The performance of the reagents is second to none. We are confident that you will find excellent data quality and even see an improvement in data quality versus many traditional frozen master mixes.
Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.
Other Products
Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
Product Info
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Product Info
Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
The kits were developed for plasmid miniprep directly from bacterial cultures. 100% centrifuge-free; NO vacuum needed; No column needed.
Plasmid MiniPrep Kit (Magnetic Beads), Cat.# 50012 – Use 2 ml of bacteria culture directly
Plasmid MiniPrep High Throughput Kit (Magnetic Beads), Cat.# 50011 – High throughput: Use 0.2 ml of bacteria culture directly with 96-well plates – Low throughput: Use 0.2 ml of bacteria culture directly with 1.5 ml tubes
The kits can be used for both high copy and low copy numbers of plasmid DNA. With BioDynami’s proprietary magnetic beads technology, the kits eliminate the requirement of centrifuge, vacuum, and column. Our magnetic beads provide a robust and reliable tool for both high throughput and low throughput applications of plasmid DNA isolation with high binding capacity and fast magnetic response time.
The High Through kit (Cat.# 50011) can be used for both high throughput and low throughput extraction of plasmid DNA. Up to 96 samples can be extracted simultaneously when using a 96-well plate.
Yield of plasmid DNA may vary dependent on the bacteria strain, plasmid type, copy numbers, and growth conditions etc. DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, molecular cloning, restriction enzymatic digestion, transfection, and transformation etc. The isolated plasmid DNA with the magnetic beads is free of contaminations such as RNA, bacterial DNA, proteins, and other impurities.
Plasmid DNA were loaded on 1% of agarose gel after extraction.
Comparison of workflows of Magnetic Beads based kits
Features
100% centrifuge-free
Culture medium can be used directly without centrifuge to pellet the bacteria
Simple
No centrifuge
No column
No vacuum
Flexible applications with simplified miniprep protocol
High throughput: 96-well plates with 0.2 ml of each culture (Cat.# 50011)
Low throughput: 1.5 ml tubes with 0.2 ml of each culture (Cat.# 50011)
Low throughput: 1.5 ml tubes with 2 ml of each culture (Cat.# 50012)
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Kit Contents
Contents
R401601
R401602
Purification Times
1 x 96
4 x 96
HiPure RNA Plate
1
4
1.5ml Collection Plate
1
4
0.5ml Collection Plate
1
4
RTL Lysis Buffer
100 ml
400 ml
RNA Binding Buffer*
30 ml
120 ml
Buffer RW1
100 ml
400 ml
Buffer RW2*
50 ml
2 x 100 ml
RNase Free Water
20 ml
80 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
