The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in animal feeds, foods and various other materials.
Detail
K-INOSL
SKU: 700004304
50 assays per kit
Content:
50 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
myo-Inositol
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
492
Signal Response:
Increase
Linear Range:
2 to 35 µg of myo-inositol per assay
Limit of Detection:
0.8 mg/L
Reaction Time (min):
~ 30 min
Application examples:
Animal feeds, food and other materials.
Method recognition:
Novel method
The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in animal feeds, foods and various other materials.
Phytic acid content of samples with very low levels of free myo-inositol can also be determined using K-INOSL. This can be achieved by measuring the amount of myo-inositol released after de-phosphorylation of phytic acid with the enzymes phytase and alkaline phosphatase, as used with the Megazyme Phytic Acid/Total Phosphorus Assay Kit (K-PHYT).
Not suitable for the determination of myo-inositol in baby formula.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
Lactobacillus/Pediococcus-Screen
Product Info
Document
Product Info
Name of Product
Lactobacillus/Pediococcus-Screen
Catalog Number
MGScLP
Short Info
This test discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 cfu/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Document
Brief Instructions The PCR reagents and the samples are prepared. After the addition of the sample to the PCR reagents, the PCR is started. The resulting PCR products are detected by a simple lateral flow test
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture
Applications
Enzyme digestion, sequencing, PCR and labeling, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Conventional plasmid, plasmid≤30KB
Sample amount
1-3ml
Elution volume
≥50μl
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
1. Suitable for extracting plasmids from 1-5ml or <3ml YT medium. 2. The same amount of buffer 1, 2, and 3 avoids errors caused by adjusting the pipette, making it convenient to use in conjunction with automated workstations. 3. Containing buffer 1 for washing, reducing the problem of false high production. 4. The purified plasmid can be directly used for sequencing, enzyme digestion, PCR, and other applications.
Kit Contents
Contents
P181102
P181103
P181104
Purification Times
100 Preps
500 Preps
5000 Preps
RNase A
10 mg
50 mg
2 x 250 mg
Buffer P1
30 ml
150 ml
2 x 750 ml
Buffer P2
30 ml
150 ml
2 x 750 ml
Buffer N3
30 ml
150 ml
2 x 750 ml
Buffer PW1
35 ml
180 ml
2 x 900 ml
MagPure Particle NB*
2.2 ml
11 ml
2 x 60 ml
Storage and Stability
RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.
Experiment Data
Document
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
