myo-Inositol Assay Kit

Description 

AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis. 

Features

• Sharp bands
• Suitable for polyacrylamide gel electrophoresis
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 2,000 bp 

Concentration 

45.5 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Room temperature for 6 months
4°C for 12 months 
-20°C for 36 months 

AccuBand™ 100 bp DNA marker II is composed of 6 individual DNA fragments, presenting 2k, 1k, 750, 500, 250 and 100 bp sharp bands respectively. This product contains 1 enhanced band (750 bp) for easy identification of bands. AccuBand™ 100 bp DNA marker II is ready-to-use, containing loading buffer with tracking dyes of dual colors (orange and cyan). To improve the faint visibility of low molecular weight bands frequently occurred in use of conventional DNA markers, AccuBand™ 100 bp DNA marker II provides sufficient amount of DNA for 250 and 100 bp fragments, and thus ensuring clear observation of all DNA bands ranging from 100 bp to 2k bp, either in agarose gel or in polyacrylamide gel electrophoresis. 

Description

The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. 

Features

• Ideal for PCR amplification and cDNA synthesis
• Premixed solution
• Nuclease and ribonuclease free

Applications 

• PCR amplification of DNA fragments
• DNA fill-in reaction
• DNA sequencing
• Reverse transcription
• One-step RT-PCR

Storage

-20°C for 36 months

The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request