The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.

Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:

– NEST 2 mL 96 Deep Well Plate, V bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 Well Plate Flat
– All Opentrons Flex tip racks

The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.

Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:

– NEST 2 mL 96 Deep Well Plate, V bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 Well Plate Flat
– All Opentrons Flex tip racks

Product Details

Application:

DNA Nucleic Acid

Format:

NFO Kit

Kit Components:

Reagents,Buffer,Enzymes

Manufacturer:

Amp-future Bio

Product Name:

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

Reaction Time:

5-20mins

Reaction Volume:

50μL

Sensitivity:

500-1000copies/ml

Shelf Life:

14 Months

Specificity:

High

Storage Conditions:

-20℃

Suitability:

Universal

High Light:

Isothermal DNA Amplification Kit

, 

DNA Amplification Kit NFO

, 

20mins nucleic acid amplification

Payment & Shipping Terms

Minimum Order Quantity

48T

Price

USD$3.8/T

Packaging Details

16T/Bag,48T/Box

Delivery Time

6 working days

Payment Terms

T/T, MoneyGram

Supply Ability

100000T/Month

Product Description

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

【Principle Overview】

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

【Primer design】

It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).

Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.

【Colloidal gold probe design】

In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.

【Features】

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.

This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.

Composition	Content
A buffer	1.6mL×1Tube
B buffer	150μL×1Tube
Positive control template-II	100μL×1Tube
Positive control probe and primer mix-II	70μL×1Tube
Reagent	48 T
Guide Manual	1 Copy

【Kit storage】

1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for the production date.

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples
Sample amount	Solid tissue: 1-10mg; Anticoagulant blood: 200μl
Elution volume	≥20μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability- handle a variety of liquid samples

Kit Contents

Contents	D301802	D301803
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	100	500
Buffer ATL	30 ml	150 ml
Buffer AL	30 ml	150 ml
Buffer GW1	22 ml	88 ml
Buffer GW2	12 ml	50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.