IST-122 QuickSeal qOpticTM Self Adhesive Sealing Film
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Adhesive film with 96 optically clear windows. This seal is peelable, suitable for qPCR and optical applications.
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Overview
Adhesive film with 96 optically clear windows. This seal is peelable, suitable for qPCR and optical applications.
These unique seals combine the strong sealing integrity of our QuickSeal PCR Seal with improved optical properties, thanks to the 96 adhesive-free windows
The seal is made of a durably transparent polyester film, and a strong adhesive is applied across the seal, apart from the 96 round windows
The QuickSeal qOptic seal is recommended for qPCR and other optical applications, such as fluorescence or colorimetric measurements
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
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ABOUT
PACE 2.0 Genotyping Master Mix ensures an unrivalled signal-to-noise ratio and produces tight data clusters, even when working with high-throughput, crude DNA preps, resulting in consistently exceptional performance. Efficiently streamline your workflow and reduce costs without compromising the quality of your results.
PACE 2.0 Genotyping Master Mix is an ideal solution for challenging starting material. PACE 2.0 has been specially formulated to overcome the obstacles presented by common PCR inhibitor compounds, such as phenols and tannins. Even notoriously tricky samples like oil palm and conifers can still be assayed using hot shot or other crude DNA prep methods and deliver reliable and accurate data.
PACE 2.0 Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE 2.0 compatible genotyping assays are comprised of two competitive allele-specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer. PACE 2.0 Genotyping Master Mix is supplied with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.
Genotyping assay designs are available from 3CR Bioscience through our free assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE 2.0 Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
For Research and Development purposes only. Not for diagnostic use.
Legal Information KASP™ is a trademark of LGC Biosearch Technologies Amplifluor® is a registered trademark of Merck KGaA
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Overview
Detection kits for HPV (High and Low Risk)
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s HPV (High and Low Risk) TaqMan PCR Lyophilized Kit is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
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Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
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