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IST-121 QuickSeal qPCR CrystalTM Self Adhesive Sealing Film

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Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.

Detail

Overview

Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.

  • Optically clear seal specifically developed for optical applications, particularly qPCR
  • It is non sticky when removed from the packaging; this aids handling when wearing gloves
  • The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
  • When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
  • For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer

Other Products

HCM001 Plate Count Agar (PCA)

Introduction

Usages:
For determination of total bacterial count .

Principle:
Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation(per liter):
Tryptone 5.0g
Yeast extract powder 2.5g
Glucose 1.0g
Agar 15.0g
Final pH 7.0 ± 0.2

How to use:
1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Saxitoxin (PSP) Lateral Flow Kit (Freshwater Streams and Source Water)

  • Screening of saxitoxin in Freshwater Streams and Source Water samples as low as 70 ppt
  • Format: 10 tests (5 tests/5 control)
  • Water Sample Bottles provided
  • Run Time: 15 Minutes

Description

Saxitoxins (STXs) are naturally occurring alkaloids produced by some marine dinoflagellates and by strains of various species of freshwater cyanobacteria.  Saxitoxin is one of the prevalent paralytic shellfish toxins (PSTs). It belongs to a family of potent neurotoxins with a molecular weight around 300 Da. Saxitoxin and its derivatives are alkaloids composed of a tetrahydropurine ring system with a highly polar guanidinium group. Due to their significant toxicity, saxitoxins are closely monitored in marine environments where they can accumulate during harmful algal blooms (HABs).  In 2009 an action level of 0.6ppb (600ppt) was recommended by EFSA as a guidance framework have been established to manage saxitoxin levels in water.

Vibrio cholerae TaqMan PCR Lyophilized Kits

Overview

  • Detection kits for Vibrio cholerae
  • Lyophilized format designed for room temperature shipping
  • Available in TaqMan format for analysis

Norgen’s Vibrio cholerae TaqMan PCR Lyophilized Kit is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Vibrio cholerae TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Figure 1 - Vibrio cholerae TaqMan PCR Kit
Figure 1 - Vibrio cholerae TaqMan PCR Kit
Figure 2 - Vibrio cholerae TaqMan PCR Kit
Figure 3 - Vibrio cholerae TaqMan PCR Kit

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Storage Conditions and Product Stability

  • All kit components should be stored at -20°C upon arrival.
  • Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
  • Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
  • All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
ComponentCat. TM38550L (100 preps)Cat. TM38510L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)4 x 350 μL
Vibrio cholerae Primer & Probe Mix (Lyo)280 μL280 μL
Vibrio cholerae Positive Control (Lyo)150 μL150 μL
Nuclease-Free Water (Negative Control)3 x 1.25 mL1 x 1.25 mL
Product Insert11