IST-113 Gas PermASeal IITM Heat Sealing Film

Name of Product

IL-6 / Interleukin-6 Test

Catalog Number

MQL6 1

Short Info

Test for the semi-quantitative evaluation of human Interleukin-6 (IL-6) in serum, plasma, cell culture supernatant, amnitotic fluid or cerebrospinal fluid.

This product is only available within the EU!

Method/Platform

lateral flow

Range/Assay Sensivity

50 to 10 000 pg/ml

Test Principle

IL-6 binds to a first anti IL-6 antibody conjugated to gold particles. The IL-6 loaded gold particles diffuse through the membrane and overflow the test line.

A second monoclonal antibody, specific for IL-6 is coated on the membrane. The gold particles are bound specifically and become visible as a coloured line. Colour intensity is directly proportional to the concentration of IL-6 in the sample. The conjugated specific antibodies printed as a second line on the membrane captures the rest of gold conjugate.

20 tests
Test for the semi-quantitative evaluation of human Interleukin-6 (IL-6) in serum, plasma, cell culture supernatant, amnitotic fluid or cerebrospinal fluid.

GL21MC high speed refrigerated centrifuge

GL21MC high speed refrigerated centrifuge Application:

Widely used in the area of animal and plant molecular biology, cell biology, clinical applications, bio-engineering, mass preparation and blood, protein precipitation, Separating the biology, viruses, sub-cell, cell and the chemicals in the cell.

Features:

1.Brushless frequency motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.

2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3.Import compressors fluorine free, double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control..
4. Microprocessor control system, the 10 inches LCD display indicates all the parameters in running,
5. There are more than 20 kinds of rotors for your choice, especial the continuous rotors which help you purify a large number of test solution continuously.
6. There are 30 kinds of program and 10 kinds of accelerating and decelerating speed for your choice.
7.ω2t centrifugation is allowed, which make you have the ideal centrifugation result.
8. 20 kinds of rotors for your choice, especial the continuous rotor help you purify a large amount of test solution continuously.
9. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
10. The centrifuge body is made of high quality steel, safe and reliable.
11. 3 tiers protection steel cover.

Technical parameters:

Max speed	21000r/min	Max RCF	46140*g
Time range	1~99H59min	Speed accuracy	±50r/min
capacity	6*500ml	Noise	≦65dBA
Weight	235KG	Dimension	865*728*1240mm
Packaged weight	285KG	Packaged dimension	1000*850*1510mm
Temperature range	-20~40℃	Temperature accuracy	±1℃
Power	AC110/220V, 50-60HZ,35A	Rotor identification	Automatic identification
Package	wooden box

Matched Rotors for GL21MC:

Order No.	Rotor	Max speed (r/min)	Max capacity (ml)	Max RCF (*g)
30108	Fixed  rotor	21000	12*10ml	44440
30117	Fixed  rotor	20000	16*10ml	46140
30111	Fixed  rotor	20000	6*50ml	44700
30118	Fixed  rotor	20000	8*30/20ml	42040
30119	Fixed  rotor	15000	8*50ml	27540
30112	Fixed  rotor	15000	6*70ml	25480
30120	Fixed  rotor	12000	8*100ml	19610
30121	Fixed  rotor	11000	4*300ml	18120
30122	Fixed  rotor	10000	6*300ml	15730
30123	Fixed  rotor	10000	6*500ml	18340
30128	Continuous centrifugal rotor	17000	1000ml	31390
30129	Continuous centrifugal rotor	14000	1800ml	21710
30130	Continuous centrifugal rotor	8000	3000ml	9450
30131	Continuous centrifugal rotor	8000	1800ml	8600
30132	Interval rotor	17000	1000ml	31390
30133	Interval rotor	12000	1200ml	19350
30134	Interval rotor	10000	3000ml	14890
30135	Vertical rotor	20000	16*5ml	38340
	Vertical rotor	18000	8*30ml	32650
30180	Swing rotor	15000	4*5ml	21670
30181	Swing rotor	13000	4*30ml	23580
30182	Swing rotor	10000	4*50ml	16240
30694	Bucket rotor	4000	4*4*96holes	2840
30696	Swing rotor	4000	4*500ml	3380

GL21M max. capacity can hold 800ml bottles 4pcs, max speed over 20000rpm
Model:
GL21M

Brand:
Yingtai

Code:
8421199090

Overview

• Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
• No sample splitting or need to use phenol or precipitation methods
• Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

RNA/DNA/Protein Purification Plus Kit

This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.

RNA/DNA/Protein Purification Plus Micro Kit

This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.

RNA/DNA/Protein Purification 96-Well Plus Kit

The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.

Details

Supporting Data

Figure 1 / 4

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Kit Specifications
Binding Capacity Per Well	50 μg for RNA 20 μg for DNA 150 μg for protein
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Size of DNA Purified	≥ 30 kb
Time to Complete 10 Purifications	35 minutes
Maximum Amount of Starting Material:Animal CellsAnimal TissuesBloodBacteriaYeastFungiPlant Tissues	1 x 106 cells10 mg100 µL1 x 109 cells1 x 108 cells40 mg40 mg
Average Yield* Liver (5 mg)	12.5 μg RNA 2 μg DNa  55 μg Protein

* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT).

Component	Cat. 47700 (50 preps)	Cat. 51600 (50 preps)	Cat. 51700 (96 preps)
Buffer SKP	40 mL	40 mL	–
Lysis Buffer Q	–	–	40 mL
Wash Solution A	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL	2 x 38 mL 1 x 18 mL
Elution Solution A	6 mL	6 mL	20 mL
Elution Buffer F	15 mL	15 mL	2 x 15 mL
Wash Solution C	30 mL	30 mL	60 mL
Binding Buffer A	8 mL	8 mL	8 mL
Elution Buffer C	8 mL	8 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Protein Loading Dye	2 mL	2 mL	3 x 2 mL
gDNA Purification Columns	50	–	–
gDNA Purification Micro Columns	–	50	–
gDNA Purification 96-Well Plate	–	–	1
RNA/Protein Purification Columns	50	–	–
RNA/Protein Purification Micro Columns	–	50	–
RNA/Protein Purification 96-Well Plate	–	–	1
Collection Tubes	150	150	–
Collection Plate	–	–	5
Elution Tubes (1.7 mL)	150	150	–
Elution Plate	–	–	3
Lysis Preparation Plate	–	–	2
Adhesive Tape	–	–	4
Product Insert	1	1	1