Virgin Polypropylene 96 Square Well plate with high uniformity from well to well.
Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-amine is a heterobifunctional reagent consisting of a propargyl group and an amine group. The amine group can form amide bonds with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl group can form triazole linkage with azides in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
High-Sensitivity RNA Amplification Kit: Fast & Accurate Results
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit Basic |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(BASIC type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from blood, buffy coat, tissue and other samples |
| Applications | Second generation sequencing, PCR, real time PCR, etc. |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Sample amount | Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg |
| Yield | 0.1 – 50μg |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | IVD3102 |
| Purification Times | 200 |
| MagPure Particles | 5 ml |
| Proteinase K | 100 mg |
| Protease Dissolve Buffer | 10 ml |
| Rnase A | 40 mg |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BW1* | 110 ml |
| Elution Buffer | 30 ml |
| Cat.No | Reagent | IVD3102-F-96 |
| Proteinase K | 50 mg | |
| Protease Dissolve Buffer | 6 ml | |
| RNase A | 20 mg | |
| Buffer ATL | 30 ml | |
| Buffer AL | 30 ml | |
| 96-Tip | 1 | |
| Sample plate (DW Plate) | 450µl Buffer BD(Ethanol Added) | 1 |
| Wash 1 Plate (DW Plate) | 600µl Buffer BW1(Ethanol Added) | 1 |
| Wash 2 Plate (DW Plate) | 600µl Buffer BW1(Ethanol Added) | 1 |
| Wash 3 Plate (DW Plate) | 750µl Buffer GW2, 20µl MagPure Particle | 1 |
| Elution plate (DW Plate) | 80µl Elution Buffer | 1 |
| Cat.No | Reagent | IVD3102-TL-06 |
| Proteinase K | 50 mg | |
| RNase A | 20 mg | |
| Protease Dissolve Buffer | 6 ml | |
| Buffer ATL | 40 ml | |
| Buffer AL | 40 ml | |
| AS-Tip | 12 | |
| 2.0ml V-bottom plate | Row 1/7：450µl Buffer BDRow 2/8：450µl Buffer BW1Row 3/9：450µl Buffer BW1Row 4/10：20μl Magpure Particle450μl Wash Buffer GW2 Row 5/11：450μl Wash Buffer GW2 Row 6/12：80µl Elution Buffer | 6 |
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
