Name of Product Megasphaera/Pectinatus Screen Catalog Number MGScMP 1 Short Info GenLine discovers beer germs in a timely and precise manner
Method/Platform PCR Range/Assay Sensivity 10^4 – 10^5 KBE/mL Test Principle The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Detail
Name of Product
Megasphaera/Pectinatus Screen
Catalog Number
MGScMP 1
Short Info
GenLine discovers beer germs in a timely and precise manner
Method/Platform
PCR
Range/Assay Sensivity
10^4 – 10^5 KBE/mL
Test Principle
The technological basis for the GenLine tests is the polymerase chain reaction (PCR) combined with lateral flow Tests.
Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.
The resulting PCR products carry the labels of the incorporated primers.
In a second part of the test, the created PCR products are detected by a lateral flow Test Strip. A “molecular sandwich” is formed and becomes visible as a line on the test Strip.
Brief Instructions
The entire procedure of the test can be divided into 3 different steps.
1.The PCR reagents and the samples are prepared.
2.After the addition of the sample to the PCR reagents, the PCR is started.
3.The resulting PCR products are detected by a simple lateral flow test Strip
Discover Huankai’s Modified MSRV Agar Base, designed for the selective detection and isolation of motile Salmonella species, ensuring reliable food and environmental testing.
Document
Discover Huankai’s Modified MSRV Agar Base, designed for the selective detection and isolation of motile Salmonella species, ensuring reliable food and environmental testing.
Usages： Determination of coliform and fecal coliform for multiple tube fermentation.
Principle: Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride osmotic pressure balance can be maintained; Lactose is a coliform fermentable sugars; potassium dihydrogen phosphate and dipotassium phosphate is a buffer; lauryl sodium can inhibit the growth of non-coliform bacteria.
How to use: 1. Suspend 35.6g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, packed in a test tube with a small down tube, 121 ℃ autoclave 15min, leave to cool to room temperature, standby . 2.Sample handling and dilution. 3.Selected three consecutive dilution, each dilution was inoculated three LST broth tubes, each tube was inoculated 1mL. 4. Put the tubes in an incubator 36 ± 1 ℃ cultured for 48 ± 2h. 5. Observe the results. if all LST broth don’t pruduse gas, can be reported as negative for E. coli, if gas production then will have to make further confirmed by experiments.
Quality control: Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows: Bacterial Name Bacterial No. Growth Status Gassing Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
