Echinococcus granulosus – IgG ELISA

Introduction

Usages:
Yeast extract is made from yeast, used in microbiological culture media as a source of nitrogen and B vitamins.

Technical specification:
Total nitrogen(TN)—————————≥10.0%
Amino nitrogen(AN) ————————≥4.0%
Moisture—————————————≤5.0%
Ash———————————————≤10.0%
Clˉ——————————————–＜2.0%


Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.

Specifications: 500g/bottle; 10kg/bag

500g/bottle; 10kg/bag

Overview

• Convenient optimized on-column DNase treatment using Norgen’s RNA Purification Kits
• Also includes protocol for digestion in-solution followed by RNA Clean-Up
• Guaranteed RNase-Free
• Includes Enzyme Incubation Buffer
• Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Details

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Introduction

Usages:
For isolation and enumeration of coagulase-positive staphylococci in foodstuffs.

Principle:
Tryptone, beef extract powder and yeast extract powder provides carbon and nitrogen sources, vitamins and growth factors; sodium pyruvate and glycine to stimulate the growth of Staphylococcus aureus; lithium chloride and potassium tellurite inhibit the non-staphylococcal microorganisms; containing lecithinase staphylococcal colonies produce degradation yolk make transparent circle, while the role of the lipase produced an opaque precipitate ring; coagulase-positive staphylococci can restore potassium tellurite and produce black colonies; agar is medium coagulant.

Formulation（per liter）:
Pancreatic Digest of Casein 10g
Beef Extract 5g
Yeast Extract 1g
Sodium Pyruvate 10g
Glycine 12g
Lithium Chloride 5g
Agar 20g
Final pH7.0 ± 0.2

How to use：
1.Suspend 63g in 950ML of distilled water , stirring heated to boiling ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Quality control：
          　　

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle
*Supplement: 029190 Egg Yolk Tellurite Emulsion

250g