Echinococcus granulosus – IgG ELISA

Overview

• Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
• Isolate total RNA, including microRNA, without phenol
• Rapid and convenient spin-column format and 96-well plate for high throughput applications
• Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Leukocyte RNA Purification Kit (Spin Column)

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 10⁶ Leukocytes

Leukocyte RNA Purification Plus Kit (Plus)

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.

Leukocyte RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 10⁶ Leukocytes.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	10 µg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small  RNA (<200 nt)
Maximum Blood Input Volume	3 mL
Time to Complete 10 Purifications	40 minutes

Storage Conditions and Product Stability 

All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 21200 (50 preps)	Cat. 21250 (50 preps)	Cat. 37800 (2 x 96 preps)
RBC Lysis Buffer	2 x 100 mL	2 x 1 L	2 x 90 mL
Buffer RL	30 mL	30 mL	–
Binding Solution	–	–	2 x 40 mL
Wash Solution A	38 mL	38 mL	–
Wash Solution	–	–	2 x 30 mL
Elution Solution A	6 mL	6 mL	–
Elution Solution	–	–	2 x 20 mL
Enzyme Incubation Buffer	–	6 mL	1
DNase I	–	1 Vial	–
Mini Spin Columns	50	–	–
Lysate Homogenization Column	–	50	–
Single Cell RNA Column	–	50	–
RBC Lysis 96-Well Plate	–	–	2
96-Well Filter Plate	–	–	2
Adhesive Tape	–	–	4
Collection Tubes	50	100	–
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	50	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	1

DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Extract high quality & quantity total RNA including miRNA 
• No phenol step required; isolate all RNA in one fraction
• Genomic DNA Removal Column for efficient elimination of gDNA
• Bind & elute all RNA irrespective of size or GC content, without bias
• Efficiently extract small RNA irrespective of GC content
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Isolate from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step. 

Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more.  Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.  Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Column	Up to 50 μg RNA
Maximum Loading Volume Per Spin Column	650 μL
Size of RNA Purified	All sizes, including < 200 nt
Time to Complete 10 Purifications	25 minutes
Maximum Amount of Starting Material
Liver Tissue	20 mg
Heart Tissue	5 mg
Kidney Tissue	20 mg
Brain Tissue	25 mg
Lung Tissue	20 mg
Spleen Tissue	20 mg
Whole Blood	100 µL
Hela Cells	3 x 106
CHO	3 x 106
Yeast	1 x 108 cells
E.coli	1 x 109 CFU/mL
Bacteria	1 x 109 cells
Plasma/Serum	200 µL
Fungi	50 mg
Plant Tissue	50 mg
Viruses	≤100 µL of viral suspension
Average Yield
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48300 (50 preps)	Cat. 48400 (100 preps)
Buffer RL	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 6 mL
RNA Purification Columns	50	100
Genomic DNA Removal Columns	50	100
Collection Tubes	100	200
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1