We will help to establish your experiments using our broad knowledge:
With myPOLS Biotec’s access to large libraries of different mutant polymerases and experience in high throughput screening we can find the best enzyme for your needs. We also optimize your polymerases for your defined requirements or enhance our DNA polymerase-based products accordingly. Finally, we can develop freeze-dried enzyme formulations and production.
Detail
We will help to establish your experiments using our broad knowledge:
With myPOLS Biotec’s access to large libraries of different mutant polymerases and experience in high throughput screening we can find the best enzyme for your needs.
We also optimize your polymerases for your defined requirements or enhance our DNA polymerase-based products accordingly.
Finally, we can develop freeze-dried enzyme formulations and production.
We at myPOLS Biotec are scientists and have vast experience in polymerase-based product development. You can benefit from this. Get in touch.
Other Products
D3139 HiPure Insect DNA 96 Kit
Product Info
Document
Product Info
Introduction
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well DNA plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D313901
D313902
Purification Times
1 x 96
4 x 96
HiPure DNA Plate
1
4
2.2 ml Collection Plate
1
4
1.6 ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Seal Film
8
32
Buffer ITL
30 ml
120 ml
Buffer IL
30 ml
125 ml
Buffer GW1
44 ml
2 x 110 ml
Buffer GW2
50 ml
3 x 50 ml
Proteinase K
50 mg
200 mg
Protease Dissolve Buffer
6 ml
15 ml
Buffer AE
20 ml
60 ml
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Document
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Document
endo-BCN-PEG2-Acrylate is a PEG linker containing a BCN group and an acrylate group. The BCN group enables copper free click chemitry with azide-tagged molecules. The acrylate group enables Michael addition reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
