40×0.5ml Angle Rotor

Introduction

Intended Use

For the enumeration of enterococci in water and other liquids by the membrane filtration technique.

Principle and Interpretation

The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.

Formulation

Ingredients	/liter
Tryptose	20.0g
Yeast extract	5.0g
Glucose	2.0g
Disodium hydrogen phosphate	4.0g
Sodium azide	0.4g
2,3,5-Triphenyl Tetrazolium chloride	0.1g
Agar	10.0g
pH 7.2±0.1 at 25°C

Preparation

Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.

Quality Control

Cultural characteristics observed after incubation at 34-38°C for 40-48hours

Quality control strains	Growth	Colony color
Enterococcus faecalis ATCC29212	Good growth,PR≥0.5	deep red coloured colonies
Escherichia coli ATCC25922	Total inhibition	–

Sorage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……

• R-RPE is manufactured from a red algae
• Protein based fluorescent particles specifically desinged for lateral flow applications
• Highly absorptive fluorescent molecule with excellent detectability

Description

R-Phycoerthyrin (R-RPE) Streptavidin Conjugate designed for Lateral Flow

Our Streptavidin R-Phycoerythrin conjugates are manufactured from a red algae and the first of its kind protein based fluorescent particles specifically desinged for lateral flow applications. R-RPE is a highly absorptive fluorescent molecule that has excellent detectability. It is the fluorochrome of choice when the brightest signal is needed and is therefore used most often when high sensitivity is essential for detectability and/or accuracy.

Consistent lot-to-lot performance resulting from continuous culture of source organisms and high purity.
– Very high water solubility
– Homogeneous structure with defined molecular weights
– Total control on growth conditions and nutrition, which avoids contamination from extraneous organisms and waste found in the open ocean. Proteins are harvested at the optimal stage of the growth cycle to assure uniform product characteristics. The pigment is extracted and stabilized within minutes of harvest, virtually eliminating risks from the action of proteases.

250ul stock ready to use for lateral flow

50mL lateral flow running buffer designed for Streptavidin R-Phycoerythrin particles 

• Excitation max: 566nm

• Emission max: 575nm

R-RPE is manufactured from a red algae

Protein based fluorescent particles specifically desinged for lateral flow applications

Highly absorptive fluorescent molecule with excellent detectability

PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.