4x50ml PRP Swing Rotor

Product Details

Application:

DNA Nucleic Acid

Format:

NFO Kit

Kit Components:

Reagents,Buffer,Enzymes

Manufacturer:

Amp-future Bio

Product Name:

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

Reaction Time:

5-20mins

Reaction Volume:

50μL

Sensitivity:

500-1000copies/ml

Shelf Life:

14 Months

Specificity:

High

Storage Conditions:

-20℃

Suitability:

Universal

High Light:

Isothermal DNA Amplification Kit

, 

DNA Amplification Kit NFO

, 

20mins nucleic acid amplification

Payment & Shipping Terms

Minimum Order Quantity

48T

Price

USD$3.8/T

Packaging Details

16T/Bag,48T/Box

Delivery Time

6 working days

Payment Terms

T/T, MoneyGram

Supply Ability

100000T/Month

Product Description

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

【Principle Overview】

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

【Primer design】

It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).

Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.

【Colloidal gold probe design】

In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.

【Features】

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.

This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.

Composition	Content
A buffer	1.6mL×1Tube
B buffer	150μL×1Tube
Positive control template-II	100μL×1Tube
Positive control probe and primer mix-II	70μL×1Tube
Reagent	48 T
Guide Manual	1 Copy

【Kit storage】

1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for the production date.

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.

t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.

Cell Culture Flask T25

Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.

Volume: 25mL75mL175mL225mL

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

• Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
• The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
• Gamma radiation sterilization
• Non-Pyrogenic, DNase/Rnase free.

Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.

Volume: 25mL75mL175mL225mL