4x10x5/7ml Swing Buckets Rotor

Introduction

Usage	For selective isolation of Staphylococcus Aureus.
How to use	Suspend 111 g in 1 L of distilled or deionized water.  Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°C for 15 minutes. Cool to 50°C and pour into sterile Petri dishes.
Formula (per liter)	Pancreatic digest of casein—————5g Pancreatic digest of animal tissue——5g Beef extract————————————1g D-mannitol————————————-10g    Sodium chloride—————————–75g Agar———————————————15g Phenolred——————————–0.025g Final pH———————————–7.4±0.2
Storage	Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Introduction

Usages:

For differentiation of enterobacteriaceae.

Principle:

Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.

Formulation(per liter):

Peptone 20g

Beef extract powder 3g

Yeast extract powder 3g

Lactose 10g

Dextrose 1g

Sodium chloride 5g

Ferric ammonium citrate 0.5g

Sodium thiosulfate 0.5g

Agar 12g

Phenol red 0.05g

Final pH 7.4 ± 0.2

How to use:

1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.

2.Diluted and treated samples.

Quality control:

Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	A/A
2	Proteus CMCC (B) 49027	Good	K/A
3	Salmonella typhimurium CMCC (B) 50115	Good	K/A

“A”Acid , “K” Alkality

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g