4x10x10ml Swing Buckets Rotor

Introduction

Usages:
For selective enrichment of Salmonella.

Principle:
Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; lactose are fermentable sugars; selenite, sodium hydrogen inhibit Gram-positive bacteria and gram-negative enterobacteria most non-Salmonella; phosphate-buffered agent; L- cystine as a reducing agent.

Formulation(per liter):
Peptone: 5g
Lactose :4g
Sodium selenite: 4g
Disodium hydrogen phosphate: 10g
L- cystine: 0.01g
Final pH 7.0 ± 0.2

How to use:
1. Weigh 23g of the product , adding 1 L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing flask, cooled to room temperature .
2. Pipette 10mL of pre-enrichment sample broth or 25mL and transferred species in the liquid sample flask in a sterile environment.
3.Place into incubator, cultured at 36 ± 1 for 18-24h.
4. Observe the results.

Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 for 18-24h ,results show as follows:
strain name strain code growth feature
Salmonella typhi CMCC (B) 50071 good red, cloudy
Salmonella typhimurium CMCC (B) 50115 good red, cloudy
Escherichia coli ATCC25922 — remain unchanged

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

500g

DL8M Largest Capacity Refrigerated Centrifuge

Features

1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.

2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. Digital display which indicates the program, speed, time, RCF, temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds off acceleration and deceleration for your choice.

5. There are 4 kinds of rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.

DL8M Technical Parameter：

Max. Speed	8000rpm
Max. RCF	14900×g
Max.   Capacity	8×2000ml(16*500ml blood bag）
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC   220V/380V 50HZ/60HZ
Dimensions   (L x W x Hmm)	940×890×1000mm /
Net   Weight(Kg)	600KG
Certificates	CE,ISO &   Calibration report are available

Matched Rotors for DL8M

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max. RCF（×g）
8M-1	Swing Rotor	4000	8 x 2000ml	5716
8M-2	Swing Rotor	4000	6×2400ml	5330
8M-3	Angle Rotor	8000	6×1000ml	14900
8M-4	Swing Rotor	4200	2×6×1000ml	5680

DL8M large capacity refrigerated centrifuge max capacity can hold 8x2000ml, can for 16pcs 500ml blood bags, can also for 8 bottles of 2000ml

Description 

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 10⁸–10⁹ cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 

Features

• Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation. 
• Fast and Easy– only few steps for preparation; suitable for time-saving transformation 
• High efficiency– up to 109 cfu/μg 
• Personalization– suitable for most E. coli strains

Kit Contents

					Component	Volume
					Champion™ CC Buffer	20 ml
					SMO-Broth™	100 ml x 2
					pUC19 Control Plasmid (10-4 μg/μl)	5 µl
					Instruction Manual	1
					Champion™ Competent Cell Preparation Card	1

Storage

4°C for 12 months

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.