4x12x10ml Swing Buckets Rotor

Overview

• Extract high quality & quantity total RNA including miRNA 
• No phenol step required; isolate all RNA in one fraction
• Genomic DNA Removal Column for efficient elimination of gDNA
• Bind & elute all RNA irrespective of size or GC content, without bias
• Efficiently extract small RNA irrespective of GC content
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Isolate from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step. 

Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more.  Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.  Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Column	Up to 50 μg RNA
Maximum Loading Volume Per Spin Column	650 μL
Size of RNA Purified	All sizes, including < 200 nt
Time to Complete 10 Purifications	25 minutes
Maximum Amount of Starting Material
Liver Tissue	20 mg
Heart Tissue	5 mg
Kidney Tissue	20 mg
Brain Tissue	25 mg
Lung Tissue	20 mg
Spleen Tissue	20 mg
Whole Blood	100 µL
Hela Cells	3 x 106
CHO	3 x 106
Yeast	1 x 108 cells
E.coli	1 x 109 CFU/mL
Bacteria	1 x 109 cells
Plasma/Serum	200 µL
Fungi	50 mg
Plant Tissue	50 mg
Viruses	≤100 µL of viral suspension
Average Yield
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48300 (50 preps)	Cat. 48400 (100 preps)
Buffer RL	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Solution A	6 mL	2 x 6 mL
RNA Purification Columns	50	100
Genomic DNA Removal Columns	50	100
Collection Tubes	100	200
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1