4x1x100ml Swing Buckets Rotor 

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

NEST 15 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Clear, printed graduations in marking area. 500 count.

Labware definition is available for immediate use in Opentron’s Labware Library.

NEST 15 mL Centrifuge Tube, clear, polypropylene tube, high-density polyethylene cap, DNase, RNase, pyrogen and endotoxin free. Sterile. Clear, printed graduations in marking area. 500 count.

Labware definition is available for immediate use in Opentron’s Labware Library.

OverView

ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market. 

Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.

This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.

For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP’s unique features continue to make it a valuable tool in various applications.

Key Features 

• Gold standard heat labile, all-purpose alkaline phosphatase.
• Completely inactivated after 5 min at 65°C or 1 min at 75°C.
• Robust: Active in most restriction enzyme buffers, no need for supplemental zinc or other additives for activity.
• Simple, hassle-free dephosphorylation of DNA, RNA, dNTPs and proteins for subsequent use in cloning or end-labelling of probes.
• No vector purification necessary.
• Easy inactivation of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis.
• Excellent stability at 4°C and RT.

Applications

• Dephosphorylation of vectors prior to cloning.
• Enzymatic clean-up of PCR products before sequencing.
• Ideal for MALDI-TOF, cloning, HLA typing, genotyping and sequencing.
• Preparing substrate in protein kinase studies.

Figures

Properties

ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.