HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
| Sample amount | 10-200μl |
| Recovery | ≥80% |
| Elution volume | ≥7μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 10µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212002 | D212003 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer DP | 60 ml | 120 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from insect tissue |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | 96 well DNA plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Insect tissue samples |
| Sample amount | |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D313901 | D313902 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure DNA Plate | 1 | 4 |
| 2.2 ml Collection Plate | 1 | 4 |
| 1.6 ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Seal Film | 8 | 32 |
| Buffer ITL | 30 ml | 120 ml |
| Buffer IL | 30 ml | 125 ml |
| Buffer GW1 | 44 ml | 2 x 110 ml |
| Buffer GW2 | 50 ml | 3 x 50 ml |
| Proteinase K | 50 mg | 200 mg |
| Protease Dissolve Buffer | 6 ml | 15 ml |
| Buffer AE | 20 ml | 60 ml |
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
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