Description

The Primerdesign genesig Kit for Shigella species (Shigella_spp) genomes is designed for the in vitro quantification of Shigella_spp genomes. The kit is designed to have a broad detection profile. Specifically,  the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.

The dynamics of genetic variation means that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions.

The target sequence is within the virulence plasmid pCP301 (VirA) which is carried by nearly all clinical isolates of shigella. This target has previously been shown to be a good genetic marker for Shigella in other real time PCR based studies (Hiroshi Fukushima et.al 2003.) The primers and probe sequences in this kit have 100% homology with over 95% of reference sequences in the NCBI database based on a comprehensive bioinformatics analysis.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

Introduction

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

This method is suitable for small quantities of tissue (<100mg) and cells (<5 X10⁶), and large quantities of tissue (up to 1g) and cells (<10⁸), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.

Details

Specifications

Features	Specifications
Main Functions	RNA isolation solvent (substitution for Trizol/Qiazol reagent)
Applications	RT-PCR, Northern hybridization, poly (a) enrichment, etc.
Purification technology	Acid phenol guanidine isothiocyanate
Process method	Manual (centrifugation)
Sample type	Animal tissue, plant tissue, adherent cells, suspended cells, bacteria, trace fungi, etc.
Sample amount	Flexible
Elution volume	Variation with sample size
Time per run	Variation with sample size

Advantages

• Extensive – suitable for various kinds of biological samples, including animals, plants, cultured cells, bacteria, etc.
• High yield – efficient cleavage releases more RNA
• High purity – purified RNA is suitable for various downstream applications
• Flexible – sample amount can be adjusted according to the demand
• High cost performance compared with similar products

Storage and Stability

MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.

Experiment Data

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.

The Opentrons Flex 96-Channel Tip Rack Adapter allows the Opentrons Flex 96-channel pipette head to be compatible with Flex tips. The adapter is placed on the work table below the tip rack to ensure the rack is secured.