Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone. Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.
Campylobacter jejuni TaqMan PCR Kit, 100 reactions
Campylobacter jejuni TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM36150 (100 preps) | Cat. TM36110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Campylobacter jejuni Primer & Probe Mix | 280 μL | 280 μL |
| Campylobacter jejuni Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx are designed for the collection and preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Devices Dx – 50 contains 50 individual Stool Nucleic Acid Collection and Preservation Devices Dx. Each Stool Nucleic Acid Collection and Preservation Device Dx consists of Norgen’s Stool Nucleic Acid Collection and Preservation Tube Dx containing Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Nucleic Acid Collection and Preservation Tube Dx is subsequently sent to the laboratory for DNA and/or RNA isolation and analysis. The stool DNA in preserved samples is stable for more than 2 years at room temperature, and the stool RNA in preserved samples is stable for 7 days at room temperature. DNA can be isolated from the preserved stool samples using Norgen’s Stool DNA Isolation Kit Dx (Cat. Dx27600) and RNA can be isolated from the preserved stool samples using Norgen’s Stool Total RNA Purification Kit Dx (Cat. Dx49500). These tubes are ideal for collecting and preserving DNA and RNA samples for in vitro diagnostic use for medical purposes.
NOTE: This product is not available for sale in the United States.
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| Kit Specifications | |
| Stool Input | 2 g |
| Stability of Stool Nucleic Acids at Room Temperature | 2 years for DNA 7 days for RNA* |
* The RNA stability will be vary depending on the samples
Stool Nucleic Acid Collection and Preservation Devices Dx – 50 Contents:
| Kit Components | Cat. Dx45660 |
|---|---|
| Stool Nucleic Acid Collection and Preservation Devices Dx | 50 |
| Product Insert | 1 |
Stool Nucleic Acid Collection and Preservation Device Dx Contents:
| Kit Components | Contents |
|---|---|
| Stool Nucleic Acid Collection and Preservation Tube Dx filled with preservative | 1 |
| Insert Card | 1 |
Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months.
Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200) and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).
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| Kit Specifications | |
| Stability of DNA at Room Temperature | 4 months |
| Stability of RNA at Room Temperature | 2 months |
Storage Conditions and Product Stability
The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.
| Kit Components | Cat. 69200 |
|---|---|
| Total Nucleic Acid Preservation Tubes | 50 |
| Product Insert | 1 |
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