These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
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| Kit Specifications | |
| Maximum DNA Input | 25 μg |
| Final Endotoxin Level | ≤ 0.1 EU/µg DNA |
| Size of DNA Purified | Up to 13,000 bp |
| Maximum DNA Volume Input | 100 μL |
| Average Recovery | > 90% |
| Time to Complete 10 Purifications | 20 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22700 (25 preps) | Cat. 52200 (10 preps) | Cat. 21900 (4 preps) |
|---|---|---|---|
| Buffer SK | 15 mL | 60 mL | 60 mL |
| Wash Solution H | 18 mL | 18 mL | 18 mL |
| Elution Buffer I | 6 mL | 12 mL | 12 mL |
| Endotoxin Removal Solution | 1.5 mL | 1.5 mL | 1.5 mL |
| Precipitation Solution | – | 1.5 mL | 1.5 mL |
| Spin Columns (assembled with Collection Tubes) | – | 10 | 4 |
| Spin Columns | 25 | – | – |
| Collection Tubes | 25 | – | – |
| Elution Tubes (1.7 mL) | 25 | – | – |
| Elution Tubes (15 mL) | – | 10 | – |
| Elution Tubes (50 mL) | – | – | 4 |
| Product Insert | 1 | 1 | 1 |
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
Figure 1 / 2
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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human
Mouse
Lynx
Wolf
Urine collected from snow
Available Sizes and Formats
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 18118 (50 tubes) | Cat. 18122 (50 tubes) | Cat. 18113 (50 tubes) | Cat. 18126 (50 tubes) | Cat. 18129 (1 cup) |
|---|---|---|---|---|---|
| Urine Collection and Preservation Tubes (5 cc) | 50 | – | – | – | – |
| Urine Collection and Preservation Tubes (15 cc) | – | 50 | – | – | – |
| Urine Collection and Preservation Tubes (50 cc) | – | – | 50 | – | – |
| Urine Preservative Single Dose | – | – | – | 50 | – |
| Urine Collection and Preservation Cup (120 cc) | – | – | – | – | 1 |
| ID Labels | 50 | – | – | 50 | – |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
Description
The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM2600 ExcelBand™ 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
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Tel : 081-875-1869 , 02-328-7179
Email : [email protected]
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