Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months.
Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200) and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).
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| Kit Specifications | |
| Stability of DNA at Room Temperature | 4 months |
| Stability of RNA at Room Temperature | 2 months |
Storage Conditions and Product Stability
The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.
| Kit Components | Cat. 69200 |
|---|---|
| Total Nucleic Acid Preservation Tubes | 50 |
| Product Insert | 1 |
TXK4 Blood card centrifuge, ID card Centrifuge, Gel card centrifuge
Application:
TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.
Features:
1. Brushless motor to make sure free maintenance,no powder polution, micro computer control and digital display the time, speed and RCF to let operation easily.
2. Special centrifugation technical to different reagent according to the requirement.
3. Regular centrifugation program, customers can press the button to choose the required one.
4. Electrical lid lock, over speed and imbalance protection.
Technical Parameters:
| Max speed | 4000rpm | Max RCF | 2250xg |
| Max volume | 24 cards | Noise | ≤45dBA |
| Timer | 0～99min | Net weight | 23KG |
| Dimension(HxDxW) | 483×320×265mm | Power supply | AC 220 50HZ 2A |
| Speed accuracy | ±10rpm | Package | carton box |
Matched Rotors for TXK4:
| Order No. | Rotor Type | Max Speed (rpm) | Max Volume (ml) | Max RCF(g) |
| NO33183 | Swing Rotor | 3000 | 24 cards | 1080 |
| 4000 | 24 cards | 2120 | ||
| NO31605 | Fixed Rotor | 4000 | 30×7/5ml | 2250 |
| NO31601 | Fixed Rotor | 4000 | 12×10ml | 2150 |
TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.
Intended Use
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
| Ingredients | /liter |
| Tryptose | 20.0g |
| Yeast extract | 5.0g |
| Glucose | 2.0g |
| Disodium hydrogen phosphate | 4.0g |
| Sodium azide | 0.4g |
| 2,3,5-Triphenyl Tetrazolium chloride | 0.1g |
| Agar | 10.0g |
| pH 7.2±0.1 at 25°C | |
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
| Quality control strains | Growth | Colony color |
| Enterococcus faecalis ATCC29212 | Good growth,PR≥0.5 | deep red coloured colonies |
| Escherichia coli ATCC25922 | Total inhibition | – |
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……