6x250ml Angle Rotor 

Introduction

Intended Use

For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.

Principle and Interpretation

MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecal Streptococci.

Formulation

Ingredients	/liter
Trypticase or tryptose	20 g
Bile salts No. 3	1.5 g
Lactose	5 g
K2HPO4	4 g
KH2PO4	1.5 g
NaCl	5 g
4-methylumbelliferyl-β-D-glucuronide (MUG)	50mg
pH6.9±0.2 at 25°C

Preparation

Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until

completely dissolved,  sterilize at 115℃ for 20min, cool to room temperature and set aside.

Quality Control

The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:

Quality control strains	Growth
Escherichia coli ATCC25922	Blue-white fluorescence is produced under 366nm UV light
Salmonella typhimurium ATCC14028	No fluorescence under 366nm UV light
Enterococcus faecalis ATCC29212	Clear, no fluorescence

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……

Description

Attogene’s 0.5mL Micro Spin Desalting Columns are convenient, simple, and ready to be used out of the box. We provide a product that facilitates equal, if not superior results to leading brands products at a significantly better cost. Superlative recovery of proteins and other macromolecules (>7000 MW) with greater than 95% retention of salts, and other small molecules (<1000 MW), are possible even with very dilute (25 ug/mL) samples. Our columns, which are constructed with a simple break away tab at the bottom, are comprised of polypropylene and contain our own proprietary resin slurry that we’ve developed in house to achieve optimal results. Sample volumes between 30-130uL can be loaded while still achieving expected purification numbers.

Download Spec Sheet

Rapid, simple, and reliable components for purification of protein samples
Cleanup before or after antibody biotinylation
Made by Attogene in the USA

DBCO-PEG4-biotin is a click chemistry biotinylation that can enable copper-free click chemistry with azide molecules to form a stable triazole linkage. PEG4 spacer increases aqueou solubility of the molecules conjugated to the biotin compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG4-biotin is a click chemistry biotinylation that can enable copper-free click chemistry with azide molecules to form a stable triazole linkage. PEG4 spacer increases aqueou solubility of the molecules conjugated to the biotin compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.