DBCO-C2-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-C2-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Character of Yingtai laboratory scale freeze dryer
Small size, and light weight
Compact construction of tabletop freeze dryer
Suitable for small samples drying
Advantage of Yingtai 3kgs smallest lab scale freeze dryer
3kg water capture capacity, which can meet the needs of general laboratory freeze-drying capacity
-70°C ultra-low temperature cold trap, which can capture conventional organic solvents
Direct-capture coil cold trap with higher efficiency and better ice-catching effect
The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry organic solvents
Large flat flange type silicone seal, effectively guarantee the vacuum tightness, no need to apply any auxiliary sealing silicone grease forever
One-button operation, the host and the vacuum pump are linked, no manual operation required
The Pirani vacuum sensor with automatic temperature compensation can accurately detect the vacuum degree in the chamber at each stage
Removable sample shelf with special holes for centrifuge tubes, making it easier to place and adjust the height
Various types of chambers and T-frame configurations can fully meet the user’s sample placement
Vacuum pumps and sample placement chambers can be freely combined according to user needs, making the configuration more flexible and convenient
Techinical parameter
| Item No | BD1-3 | BD1-6 | BD2-6 |
| 24/h water capture capacity | 3kg | 6kg | 6kg |
| Cold trap temperature | -70℃ | -70℃ | -90℃ |
| Cold trap volume | 5L | 10L | 10L |
| Freeze dried chamber | High permeability organic glass/multi manifold external valve/stainless steel chamber | ||
| Sample shelf | φ 200mm stainless steel with 3-5 layers available | ●Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional | |
| vacuum pump | Standard configuration | ||
| Oil mist filter | Extreme vacuum 4 * 10-4mbar,with a pumping capacity of 78L~200L/optional pump of various types | ||
| Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | ||
| anti-corrosive treatment | Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | ||
| External valve | 6/optional | 1-48 pieces/optional | |
| Eutectic point detection system | Support/Optional | ||
| Data output analysis | Support/Optional | ||
| Power | 0.6kw | 1.1kw | 0.5kw |
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 10 µg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Blood Input Volume | 3 mL |
| Time to Complete 10 Purifications | 40 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 21200 (50 preps) | Cat. 21250 (50 preps) | Cat. 37800 (2 x 96 preps) |
|---|---|---|---|
| RBC Lysis Buffer | 2 x 100 mL | 2 x 1 L | 2 x 90 mL |
| Buffer RL | 30 mL | 30 mL | – |
| Binding Solution | – | – | 2 x 40 mL |
| Wash Solution A | 38 mL | 38 mL | – |
| Wash Solution | – | – | 2 x 30 mL |
| Elution Solution A | 6 mL | 6 mL | – |
| Elution Solution | – | – | 2 x 20 mL |
| Enzyme Incubation Buffer | – | 6 mL | 1 |
| DNase I | – | 1 Vial | – |
| Mini Spin Columns | 50 | – | – |
| Lysate Homogenization Column | – | 50 | – |
| Single Cell RNA Column | – | 50 | – |
| RBC Lysis 96-Well Plate | – | – | 2 |
| 96-Well Filter Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Collection Tubes | 50 | 100 | – |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 50 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 1 |