12x10ml Angle Rotor 

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Applications

• Viral Bioprocessing
• Complete Removal of DNA

M-SAN HQ  – Peak performance where it matters most at physiological conditions

Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.

This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations between 125 – 250 mM. Due to its excellent performance at physiological conditions, M-SAN HQ can be used directly in the cell medium or the harvested supernatant without buffer adjustments. This makes M-SAN HQ ideal for the manufacturing of fragile vectors such as lentiviruses and retroviruses.

M-SAN HQ can be directly used in medium without buffer adjustments The high activity of M-SAN HQ at standard cell medium conditions leads to improved DNA clearance compared to other commonly used nucleases. In the data (see Figure 6), an over 2-fold reduction in residual DNA was achieved. 

Key Benefits

• Compatibility: Ideal for working with both fragile and robust viral vectors and proteins in a variety of cell media
• Optimum activity: Optimization for cell media salinity allows both shorter DNA fragments and reduced incubation times.
• Cost-Effectiveness: Reduced need for additional reagents and steps can lower production costs
• Quality: Maintains the integrity of sensitive or labile biological molecules, ensuring a higher quality end product
• Flexibility: Can be used directly in a variety of media without the need for customization

Key Features 

• High purity (≥ 99%)
• No protease detected
• Endotoxin-tested
• Animal origin-free production
• Supplied with extended product documentation

Adapted to use in medium without salinity adjustments 

Optimal activity at physiological salinity and pH makes it ideal for DNA removal from mammalian cell media. (see Figures)

The high activity of M-SAN HQ at the physiological salinity and pH found in standard cell medium conditions leads to improved DNA clearance compared to commonly used nucleases.

Simple to Optimize in Cell Media

M-SAN HQ is uniquely formulated to excel in physiological pH conditions, offering high performance at the commonly used cell media pH of 7.4.

While other nucleases often require more alkaline environments, M-SAN HQ stands out for its adaptability and effectiveness in cell media. It’s the nuclease that truly aligns with your bioprocessing needs. (See Figure section)

M-SAN HQ ELISA Kit

The M-SAN HQ ELISA Kit confirms the removal of M-SAN High Quality in bioprocessing and biomanufacturing applications with high accuracy: 

• 100% (±10%) Measuring range 0.47 – 7.50 ng/ml
• 100% (±20%) Measuring range 0.12 – 0.47 ng/ml

Features:

Shelf life: 18 months

Fast: 1.5 – 2 hours

Sensitive – Quantification range: 0.12 – 7.5 ng/ml

Accurate

Flexible – 96-well plate in 8-well strip format

Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.

TGL18 Tabletop 21000rpm High Speed large capacity Swing out rotor 4x800ml universal Refrigerated Centrifuge Adapters for 5/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.

TGL18 Universal Refrigerated Centrifuge Application:

Holding lots of Swing Out Rotors & Angle Rotors for options. Mainly used in animal and plant molecular biology, cell biology, food safety, life science, agriculture and forest science, animal husbandry science, biological products, pharmaceutical products and other fields of ideal equipment for the separation and precipitation of samples preparation.

TGL18 Universal Refrigerated Centrifuge Features:

1. With silent-block and shock absorbers that guarantees smooth and quiet operations & fast cooling.

2. The centrifuge body is made of high-quality steel, safe and reliable.

3.  Microprocessor digital control of all functions:speed, time,

temperature, acceleration/deceleration, RCF, 10 program memory, operation fault display.

4. Electronic safety lock alarm inside, just press down the top lid until it will lock in sealed and open up after separation automatically.

5. Easy open the centrifuge lid via pneumatic springs; the operation area of the centrifuge is at the front end.

6. RPM/RCF adjustable along with the speed setting calculating automatically.

7. 10 selective acceleration and deceleration rates for options

8. The self-diagnostic system provides a protection for imbalance over speed/voltage and electronic lock fault.

TGL18 Universal Refrigerated Centrifuge Technical Parameters:

TGL18 Universal Refrigerated Centrifuge Matched Rotors:

The Corners of our Company____________________________________

Metal housing with 3-tiers protection. It can hold both swing out rotors and angle rotors in low speed and high speed spining to switch smoothly. And its Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.

DBCO-PEG4-PC represents a photocleavable, PEG linker consisting of a DBCO group that can be used for copper-free Click Chemistry reactions. This structure allows for efficient photorelease of linked molecules, typically achieving over 90% cleavage in 5-25 minutes using a near-UV lamp. The PEG4 spacer increases the hydrophilicity of the compound.

DBCO-PEG4-PC represents a photocleavable, PEG linker consisting of a DBCO group that can be used for copper-free Click Chemistry reactions. This structure allows for efficient photorelease of linked molecules, typically achieving over 90% cleavage in 5-25 minutes using a near-UV lamp. The PEG4 spacer increases the hydrophilicity of the compound.