Introduction

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.

Details

Specifications

Features	Specifications
Main Functions	Isolation gDNA from biological sample and remove host DNA
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture medium, swab, parasitic blood, tissue, sputum, etc.
Sample amount	Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, etc.:0.5-1ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica Column purification. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High concentration – the host DNA is digested by dnase, without contamination of host DNA
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time
• Wide applicability – it can be used in blood, tissue, intestinal contents and other samples

Kit Contents

Contents	D314802	D314803
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	50	2 x 125
2ml beads Tubes	50	250
Buffer DRB	15 ml	60 ml
Buffer ES	6 ml	30 ml
Reagent DX	0.5 ml	1 ml
Buffer DL	30 ml	120 ml
Buffer GW1	22 ml	110 ml
Buffer GW2	12 ml	50 ml
DNase I (Powder)	6 mg	30 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml

Storage and Stability

DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.

Safety Features

1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.

2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.

3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.

4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.

5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.

6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.

7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.

8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.

9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.

10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.

11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.

Technical Parameters

Model	HB65A Standard TypeHB65B  Auto Water Replenishment TypeHB65C Auto Drying Type	HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type	HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type
Capacity	65L	90L	110L
Dimensions (L × W × H) mm	664×660×994.5	664×660×994.5	664×660×1200
Sterilization Chamber Dimensions (Diameter × Height) mm	φ400×530	φ400×730	φ400×920
Rated Power	2800W	3400W	4500W
Rated Voltage	220V±10%50Hz
Temperature Display Accuracy	0.1℃
Operating Environment	5℃~40℃、Relative Humidity10%～85%
Sterilization Chamber Material	SUS304Stainless Steel
Sterilization Working Temperature	105℃~143℃
Sterilization Time Pre-set Range	1minute ～9999minutes
Melting Temperature Pre-set Range	60℃~100℃
Melting Time Pre-set Range	1minute～9999minutes
Insulation Temperature Pre-set Range	45℃~60℃
Insulation Time Pre-set Range	1minute～9999minutes
Door Opening Temperature Pre-set Range	Solid, Agar, and Waste Modes: 40°C ~ 99°C        Liquid Mode: 40°C ~ 80°C
Clock FunctionTimer Pre-set Range	Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code.
Pressure Gauge Display Range	0～0.6Mpa
Pressure	Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department.
Pressure Safety Protection	Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection
Sterilization Modes	·  Solid Mode: Heating → Sterilization → Exhaust, Air Cooling·  Solid with Drying Mode: Heating → Sterilization → Drainage, Exhaust → Drying·  Liquid Mode: Heating → Sterilization → Pre-cooling → Exhaust·  Liquid with Insulation Mode: Heating → Sterilization → Pre-cooling → Exhaust → Insulation·  Waste Mode: Heating → Sterilization → Exhaust, Air Cooling·  Agar Mode: Heating → Melting → Insulation
Exhaust Control and Water Tank	Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank
Cover Opening Method and Interlocking Device	Unique eight-column interlocking device, flip-cover switch, touch-type handle
Controller and Memory Storage Function	Microcomputer intelligent control system. 6 fixed operation modes, 82 modifiable modes, 88 memory storage programs
Safety Devices	Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system
Accessories	Condensate collection bottle, water separation plate, drainage hose, stainless steel basket
Optional Accessories	Printer, cooling fan, item temperature sensor, USB output
Stainless Steel Baskets	(φ360×230)1pcs(φ360×200)1pcs	(φ360×200)1pcs(φ360×400)1pcs		(φ360×200)2pcs(φ360×400)1pcs

Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.

Other Optional Accessories

1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.

2. Stainless Steel Basket with Base.

3. Pressure Sensor

4. Stainless Steel Bucket

5. Item Thermometer

6. 3Q Validation Adapter

Features and functions:

1. Large-diameter sterilization chamber：The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.

2. Sterilization temperature：105-138℃.

3. Exhaust control ：After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.

4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.

5. Saturated steam monitoring：The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.

6. Pressure performance：The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.

7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.

8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.

9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.

10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.

11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.

12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.

13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.

Model:
HB65/ HB90/ HB110