High Purity DNA Purification Kit

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12 Months DNA Purification Kit

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Nucleic Acid dna isolation kit

Product Description

Rapid Nucleic Acid Release Reagent (DNA) – Type II

High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids

Product parameters:

 Product features and advantages:

 Product application:

  It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.

  Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.

Overview

Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.

• Crystal clear seal specifically developed for optical applications, particularly qPCR
• It is non sticky when removed from the packaging; this aids handling when wearing gloves
• The adhesive is contained within small capsules, allowing light to pass through to ensure the optical clarity of the seal
• When the seal is in position, pressure can be applied to burst the capsules, releasing a strong adhesive only where the seal touches the raised well rims of the plate – the rest of the seal area above the wells remains optically clear
• For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer

Crystal clear adhesive film which benefits from pressure activated adhesive. This seal is DMSO resistant, peelable; suitable for qPCR and other imaging techniques including crystallization.

Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Details

Specifications

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• Low elution volume – elution volume can be as low as 10μl
• General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.