12x15ml Angle Rotor 

Product Description

Lyophilized Diagnostic Test Kit – Convenient Accurate And -20°C Ready

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

• Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
• A total of 480 strips are provided
• 100 cassettes
• 125mL of buffer

Description

Blank lateral flow strips are the perfect companion during the development and feasibility testing of a lateral flow device.  IN THE BEGINNING, the capture and test line reagents can be spotted onto the nitrocellulose portion of the blank strips at different concentrations, volumes and buffer conditions.  This enables the user to determine the optimal conditions for subsequent spraying and enables the user to verify the test and controls will respond according to expectations before scaling up the process.

These blank strips are compatible with all of our colloidal gold products AND:

Lateral flow cassettes: C02022

Streptavidin CdSe/ZnS Quantum Dots For Lateral Flow: AU2043

Streptavidin Europium Chelate Microspheres For Lateral Flow: AU2050

Streptavidin Colloidal Gold For Lateral Flow: AU2017

RNase-Free Lateral Flow Assay Running Buffer Screening Pack: AU2035

Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
A total of 480 strips are provided
100 cassettes
125mL of buffer

Overview

• Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive
• Bead tubes (provided) allow for effective mechanical homogenization
• Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more

These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.

Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)

This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.

Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)

Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.

Details

Supporting Data

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Kit Specifications
Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Fungi (wet weight) Yeast or Bacteria Culture	50 mg 0.5mL – 1mL
Average Yield* Pichia sp. (yeast) Botrytis cinerea ​Fusarium sp. Aspergillis niger Mucor racemosus Cladosporium cladosporioides Penicillium sp. Rhizopus oryzae Alternaria tenuissima	25 μg 32 μg 42 μg 26 μg 15 μg 12 μg 40 μg 7 μg 30 μg
Time to Complete 10 purifications	30 minutes

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 27300 (50 preps)	Cat. 27350 (192 preps)
Lysis Buffer L	30 mL	2 x 60 mL
Resuspension Solution A	20 mL	60 mL
Solution BX	28 mL	2 x 28 mL
Wash Solution A	18 mL	2 x 38 mL
Elution Buffer B	15 mL	30 mL
Bead Tubes	50	200
Spin Columns	50	–
Collection Tubes	50	–
96-Well Plate	–	2
96-Well Collection Plate	–	2
Adhesive Tape	–	4
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Product Insert	1	1