The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| Applications | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Sample amount | Cells: ≤1 x 107 Animal tissue: 1-20 mgPlant leaves: 50-150 mg Yeast cells: 5 x 106 |
| Yield | 2-100μg |
| Elution volume | ≥50μl |
| Time per run | ≤15 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Advantages
Kit Contents
| Contents | R401102 | R401103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| RTL Lysis Buffer | 50 ml | 200 ml |
| RNA Binding Buffer | 15 ml | 75 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
DBCO-C2-alcohol is a linker containing a DBCO moiety and a terminal primary hydroxyl group. DBCO group can react with azides in copper-free Click Chemistry reactions. The hydroxyl can react with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-C2-alcohol is a linker containing a DBCO moiety and a terminal primary hydroxyl group. DBCO group can react with azides in copper-free Click Chemistry reactions. The hydroxyl can react with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DD6 Floor Standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine
Application
for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine
Widely used in the area of laboratory, pharmaceutical factory, biochemistry, biological products and so on. It is the ideal instruments for blood separation, protein precipitation and cell collection.
Features
for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine
1. Brushless DC motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
4. Microprocessor control, speed, time, temperature and RCF in operation, speed rising and reducing quick, operate simply.
5.10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
6. There are many rotors for your choice, adapters are available by experiment requirements.
7.3 tiers protection steel cover, safe and reliable.
Technical Parameters
for 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine
| Max Speed: 6000r/min | Max.RCF: 6600xg |
| Max Volume: 4x1000ml | Power supply:AC220V,50HZ,10A |
| Timer: 1~99min | Noise:≤55dBA |
| Dimension:800×645×830mm | Net Weight: 170KG |
| Speed Accuracy: ±20r/min | Package(W*D*H): 920×800×1320mm |
| Temperature range: | Inapplicable |
Matched Rotors for DD6 laboratory centrifuge
| Order No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
| No33097 | Swing out rotor | 4,000 | 4x1000ml (round/square cup ) | 4,060 |
| No30221 | Angle rotor | 6,000 | 4x300ml | 5,390 |
| No30222 | Angle rotor | 6,000 | 6x300ml | 5,660 |
| No30223 | Angle rotor | 6,000 | 6x500ml | 6,600 |
Packing & Shipping_____________________
by Sea/ by Air / by Express Sevices DHL, Fedex, TNT…
DD6 is floor standing large capacity 4x1000ml, 6x300ml, 6x500ml laboratory centrifuge machine without refrigerated one. It is up to max.speed 6000rpm.