Overview

• Detection kits for the JCV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The JC virus (JCV) is a type of human polyomavirus and belongs to the family Papovaviridae. JCV is a double-stranded DNA virus, and is genetically similar to BK virus and SV40. The virus is very common in the general population and it is believed that most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in children with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. However, it has not been established whether PML is the result of a primary infection with JCV in a person with impaired immunity or whether it follows reactivation of latent virus. JC virus is also the primary cause of nephropathy (kidney disease) in people who have received a kidney transplant and are on immunosuppressive therapy.

JCV TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

JCV TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

Figure 1 / 3

Previous

Next

Click for expanded view

Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM37250 (100 preps)	Cat. TM37210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
JCV Primer & Probe Mix	280 μL	280 μL
JCV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount	Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 20 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time

Kit Contents

Contents	D312502	D312503
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	60 ml
Buffer AL	15 ml	60 ml
Buffer GW1*	22 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Carrier RNA	310 μg	2 x 310 µg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml
HiPure DNA Mini Columns I	50	2 x 125
2 ml Collection Tubes	100	5 x 100

Storage and Stability

Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

Experiment Data

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

Introduction

Place of Origin: Guangdong, China

Warranty: 2 years

Customized support: OEM

Brand Name: HKM

Model Number: CRM012

Reagent grade: Biochemical Reagent

Form: Powder

Color: Light yellow

Type: Staphylococcus Aureus Agar

1000mL