Propargyl-PEG14-acid

Overview

• Fast, reproducible and easy processing of samples
• High yield and high quality genomic DNA with no RNA or protein contamination
• DNA ready for any application including PCR, qPCR, genotyping and more

Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.

Cells and Tissue DNA Isolation Kit (Spin Column)

Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Micro Kit (Micro)

Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.

Cells and Tissue DNA Isolation Kit (Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.

Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 9

Previous

Next

Click for expanded view

Kit Specifications
Maximum Cells and Tissue Input	20 mg of animal tissue 3 x 106 cells
Average Yield*	8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells)
Average Purity (OD260/280)	1.8 – 1.9
Time to Complete 10 Purifications	40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. 53100 (50 preps)	Cat. 57300 (50 preps)	Cat. 59100 (50 preps)	Cat. 62500 (192 preps)
Lysis Buffer B	20 mL	20 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	18 mL	18 mL	55 mL
Wash Solution A	18 mL	18 mL	–	–
Elution Buffer B	30 mL	8 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K	1.2 mL	1.2 mL	1.2 mL	–
Proteinase K in Sotrage Buffer	–	–	–	4 mL
Magnetic Bead Suspension	–	–	2 x 1.1 mL	8.5 mL
96-Well Plate	–	–	–	2
Spin Columns	50	–	–	–
Micro Spin Columns	–	50	–	–
Collection Tubes	50	50	–	–
Elution Tubes (1.7 mL)	50	50	50	–
96-Well Elution Plate	–	–	–	2
Adhesive Tape	–	–	–	2
Product Insert	1	1	1	1

K-GLUM

SKU: 700004300

50 Assays per kit

Content:	50 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Glucomannan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 μg of glucomannan per assay
Limit of Detection:	1 g/100 g
Total Assay Time:	120 min
Application examples:	Jelly sweets, cosmetics, food gums and other materials.
Method recognition:	Novel method

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

View more of our polysaccharide test kits.

Advantages

• Very cost effective 
• Only enzymatic kit available 
• Simple format 
• All reagents stable for > 2 years after preparation 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

PEG13-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a large PEG linker. The alkynes are most frequently used in copper click chemistry with azides. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter ADME of this compound.

PEG13-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is large molecule with two sets of three terminal alkynes joined together by a large PEG linker. The alkynes are most frequently used in copper click chemistry with azides. The PEG linkers as well as numerous amide bonds provide high aqueous solubility to this compound, which may alter ADME of this compound.