Propargyl-PEG8-NHS ester

• R-RPE is manufactured from a red algae
• Protein based fluorescent particles specifically desinged for lateral flow applications
• Highly absorptive fluorescent molecule with excellent detectability

Description

R-Phycoerthyrin (R-RPE) Streptavidin Conjugate designed for Lateral Flow

Our Streptavidin R-Phycoerythrin conjugates are manufactured from a red algae and the first of its kind protein based fluorescent particles specifically desinged for lateral flow applications. R-RPE is a highly absorptive fluorescent molecule that has excellent detectability. It is the fluorochrome of choice when the brightest signal is needed and is therefore used most often when high sensitivity is essential for detectability and/or accuracy.

Consistent lot-to-lot performance resulting from continuous culture of source organisms and high purity.
– Very high water solubility
– Homogeneous structure with defined molecular weights
– Total control on growth conditions and nutrition, which avoids contamination from extraneous organisms and waste found in the open ocean. Proteins are harvested at the optimal stage of the growth cycle to assure uniform product characteristics. The pigment is extracted and stabilized within minutes of harvest, virtually eliminating risks from the action of proteases.

250ul stock ready to use for lateral flow

50mL lateral flow running buffer designed for Streptavidin R-Phycoerythrin particles 

• Excitation max: 566nm

• Emission max: 575nm

R-RPE is manufactured from a red algae

Protein based fluorescent particles specifically desinged for lateral flow applications

Highly absorptive fluorescent molecule with excellent detectability

Overview

• Compatible with PAXgene Blood RNA Tubes
• Isolate true total RNA including siRNA and microRNA
• No phenol extractions
• Purify high-quality RNA in 30 minutes
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.

* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH.  PAXgene Blood RNA Tubes are not provided.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	30 minutes
Average Yield	5 – 20 μg per 2.5 mL preserved human blood

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C.

Component	Cat. 43500 (50 preps)
NPX1	2 x 110
NPX2	40 mL
NPX3	22 mL
NPX4	6 mL
NPX5	6 mL
DNase I	1 vial
Mini Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

OverView

ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.

Key Features:

• Novel substrate specificity
• RNA to DNA ligation
• High purity
• Detergent free
• Efficient ligation of DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the ligatable ends of DNA and RNA
• ATP dependent dsDNA ligase
• Utilizes Mg²⁺ or Mn²⁺

Suggested Applications:

• RNA capturing
• In vitro transcription  
• NGS library prep
• Transcriptome amplification
• miRNA detection/analysis
• RNA splint ligation

ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.