Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Details

Specifications

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
• High purity – can be used for downstream applications such as multiplex and quantitative PCR
• Fast – simplified process, extracting several samples in 20 minutes (after digestion)
• Safety – no phenol orchloroform extraction, no alcohol precipitation

Kit Contents

Storage and Stability

RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Specification

7-50x    continuous    zoom    stereo    microscope		XTL-205A	XTL-205B	XTL-204A	XTL-2048	XTL-203A	XTL-203B
Zoom      Body	Binocular head,45°decline,360°rotate	●		●		●
	Trinocular head,45°decline,360*rotate		●		●		●
Eyepiece	WF10X22mm	●	●	●	●	●	●
Objective	zoom objective 0.7X—5X	●	●	●	●	●	●
Zoom     Rate	0.1:07.1	●	●	●	●	●	●
Total Amplific- ation   Rate	the standard configuration is 7X-50X	●	●	●	●	●	●
Auxiliary Lens	0.5X,0.7X,1X,1.5X,2X For Option	O	O	O	O	O	O
WorkingDistance	100mm	●	●	●	●	●	●
Diopter	55mm-75mm	●	●	●	●	●	●
Focus    arm	76mm Diameter,32mm pole size	●	●	●	●	●	●
Plate	Black/White plate,Glass plate for option	●	●	●	●	●	●
Optional	Stand	Q	Q
Stand	BI3 base.column sunnort unner and ower light source 3 WLED			●	●
	BL3-A base vertical arm bracket upper and lower light source 3 WLED	●	●
	B3 base column support has no light source					●	●
External light source	LED illumination	Q	Q	Q		O
Optiona	Photography,camera accessories CCD Coupler	o	o	o	o	o	o
Optional	Display attachment Monitor

N-(Propargyl-PEG2)-N-Boc-PEG3-t-butyl ester is a click chemistry branched linker. The propargyl group can react with azide-bearing compounds through Click Chemistry, the t-butyl protected carboxyl group can also be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Propargyl-PEG2)-N-Boc-PEG3-t-butyl ester is a click chemistry branched linker. The propargyl group can react with azide-bearing compounds through Click Chemistry, the t-butyl protected carboxyl group can also be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.